Abstract：A technique based on Fourier transform infrared (FTIR) spectrometry was used to detect the physiological changes in chemical composition of Phytolacca americana under a wide range of Mn2+ treatments (0,0.125,0.25,0.5 and 1 g·kg-１). Using the roots,stems and leaves of phytolacca americana,absorption bands corresponding to carbohydrates,ester and proteins varied differently. The absorption band heights at 3 000 and 2 916 cm-1 of stem tissues rose firstly and then decreased,indicating the exudation and transporting situation of organic substances which served as organic osmotic contents to enhance manganese tolerance under low treatments; Meanwhile,the band heights at 2 922 cm-1(roots) and 1 606 cm-1 (leaves) which have different tendency in accordance with Mn treatments showed that the capability to chelate Mn decreased under higher manganese concentration. In addition,the changes of the bands at 1 732 and 1 026 cm-1 (roots),1 028 cm-1 (stems),1 052 and 967 cm-1 (leaves) differed from each other,suggesting that under the conditions of severe Mn stress the ex-oxidation of membrane lipid increased; Otherwise,the band heights at 1 375 cm-1 increased firstly and then decreased,representing that the cell wall improved Mn resistance by increasing cation-exchange capacity(CEC). The result also implied that it is practical to apply FTIR to the research comparing the chemical differences of phytoaccumulators under metal treatments.
任立民1,2,成则丰1,刘鹏1*,李志刚2. 美洲商陆对锰毒生理响应的FTIR研究[J]. 光谱学与光谱分析, 2008, 28(03): 582-585.
REN Li-min1,2,CHENG Ze-feng1,LIU Peng1*,LI Zhi-gang2. Studies on the Physiological Response of Phytolacca Americana to Manganese Toxicity by FTIR Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(03): 582-585.
