Effects of Environmental Factors and Denaturants on the Specific Absorption Spectrum and Fluorescence Characters of B-Phycoerythrin
HU Jin-mei1, WEI Dong1*, GUO Si-yuan1, CHEN Feng1,2
1. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China 2. Department of Botany, University of Hong Kong, Hong Kong，China
Abstract：Effects of environmental factors and denaturants on the variation rules of the specific absorption spectrum and fluorescence emission intensity of B-phycoerythrin were systematically investigated in the present paper. The results showed that B-phycoerythrin was relatively stable under certain conditions: the pH range from 4.5 to 9.5, temperature range from 25 to 60 ℃, and illumination intensity at 2 800 lx in 24 h. The specific absorption spectrum and fluorescence emission intensity of B-phycoerythrin changed slightly. The conformation of B-phycoerythrin changed remarkably with 0.5％-3％ SDS or 0.25％-1％ β-mercaptoethanol, the fluorescence emission intensity declined greatly, and the specific absorption peaks disappeared. The fluorescence emission intensity declined, while the specific absorption spectrum did not change, but the peak intensity reduced slightly with 10％-70％ acetonitrile or 2-8 mol·L-1 urea. The possible reasons for the conformation changes of B-phycoerythrin with various environmental factors and different denaturants were briefly discussed.
胡金梅1,魏东1*,郭祀远1,陈峰1,2. 环境因子和变性剂作用下B-藻红蛋白的光谱与荧光特性[J]. 光谱学与光谱分析, 2006, 26(12): 2258-2263.
HU Jin-mei1, WEI Dong1*, GUO Si-yuan1, CHEN Feng1,2 . Effects of Environmental Factors and Denaturants on the Specific Absorption Spectrum and Fluorescence Characters of B-Phycoerythrin. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(12): 2258-2263.
[1] CHEN Feng，JIANG Yue(陈 峰, 姜 悦). Microalgae Biotechnology(微藻生物技术). Beijing: China Light Industry Press(北京: 中国轻工业出版社), 1989. 108. [2] LI Guang-wu，WANG Guang-ce, LU Lei，et al(李冠武, 王广策, 陆 雷, 等). Acta Laser Biology Sinica(激光生物学报), 1997, 6(3): 1119. [3] Glazer A N. Appl. Phycobiliprotein, 1994, 6: 104. [4] Sachurfu, LUO Liao-fu(萨楚尔夫, 罗辽复). Acta Scientiarum Naturalium Universitatis Neimongol(内蒙古大学学报·自然科学版), 2003, 34(1): 47. [5] HUANG Yan, QIN Li, ZHU Jin-chang(黄 岩, 秦 利, 朱晋昌). Photographic Science and Photochemistry(感光科学与光化学), 1993, 11: 56. [6] Johes R F, Speer H L, Kury W. Physiologia Plantarum, 1963, 16: 636. [7] LIU Shi-sheng, WEI Dong, ZHU Gui-fang(刘石生，魏 东，朱桂芳). Journal of South China Univ. of Tech.(Natural Science Edition)(华南理工大学学报·自然科学版), 2005, 33(2)：82. [8] Bermejo Roman R, Alvarez-Pez J M, Acien Fernandez F G. Journal of Biotechnology, 2002, 93: 73. [9] Richard G Fisher, Nancy E Woods, Herbert E Fuchs. Journal of Biotechnology, 1980, 255(11): 5082. [10] Daniel J Lundell, Alexander N Glazerg. Journal of Biological Chemistry, 1984, 259(9): 5472. [11] Ruperto Bermejo, Diego J Tobaruela, Eva M Talavera, et al. Journal of Colloid and Interface Science, 2003, 263: 616. [12] Vaidya S, Orta-Ramirez A, Smith D M, et al. Journal of Biotechnology and Bioengineering, 2003, 4: 465. [13] Galland-Irmouli A V, Pons L, Lucon M, et al. Journal of Chromatography B, 2000, 739: 117. [14] Glazer A N. Mol. Cell Biochem., 1977, 18: 125. [15] YANG Cang-zhen, MA Jin-shi, JIANG Li-jin(杨苍珍, 马金石, 蒋丽金). Progress in Biochemistry and Biophysics(生物化学与生物物理进展), 1988, 15: 200. [16] CHEN Xiao-qiang, GONG Xing-guo, SHI Feng(陈小强, 龚兴国, 史 锋). Chinese Journal of Analysis Laboratory(分析试验室), 2004, 23(11): 5. [17] LIANG Hong, SONG Zhong-rong, ZHOU Yong-qia, et al(梁 宏，宋仲容，周永洽, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 1994, 14(6): 39. [18] ZHENG Jiang, GAO Ya-hui , WANG Wen-xing(郑 江, 高亚辉, 王文星). Journal of Xiamen University(Natural Science)(厦门大学学报·自然科学版), 2003, 42(1): 117. [19] ZHANG Jing(张 静). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(4): 455. [20] WU Hua-ju, ZHANG Jian-ping, XIA An-dong(伍华菊, 张建平, 夏安东). Photographic Science and Photochemistry(感光科学与光化学), 1995, 13(1): 53. [21] HE Jin-an, YE Xi-lin, JIANG Li-jin(何靳安，叶锡林，蒋丽金). Acta Biochimica et Biophysica Sinica(生物化学与生物物理学报), 1994, 26(4): 371. [22] ZHANG Xi-ying, LIU Lu-ning, CHEN Xiu-lan, et al(张熙颖, 刘鲁宁, 陈秀兰, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(10): 1224. [23] YANG Fang, LIANG Yi(杨 芳, 梁 毅). Acta Chimic Sinica(化学学报), 2003, 61(6): 803.
