Detection of Erucic Acid and Glucosinolate in Intact Rapeseed by Near-Infrared Diffuse Reflectance Spectroscopy
RIU Yu-kui1, HUANG Kun-lun1, 2, WANG Wei-min3, GUO Jing1, JIN Yin-hua1, LUO Yun-bo1*
1. Laboratory of Food Technology of China Agricultural University，Beijing 100083, China 2. Supervision Inspection and Test Center of Farm Products of Agricultural Ministry, Beijing 100083, China 3. Center of Science and Technology of Agricultural Ministry, Beijing 100026, China
Abstract：With the rapid development of transgenic food，more and more transgenic food has been pouring into the market, raising great concern about transgenic food’s edible safety. To analyze the content of erucic acid and glucosinolate in transgenic rapeseed and its parents, all the seeds were scanned intact by continuous wave of near infrared diffuse reflectance spectrometry ranging from 12 000 to 4 000 cm-1 with a resolution of 4 cm-1 and 64 times of scanning. Bruker OPUS software package was applied for quantification, while the results were compared with the standard methods. The results showed that the method of NIRS was very precise, which proved that infrared diffuse reflectance spectroscopy can be applied to detect the toxins in transgenic food. On the other hand, the results also showed that the content of erucic acid in transgenic rapeseeds is 0.5-1.0 times higher than that of their parents, and the content of glucosinolate in transgenic rapeseeds is 1.3-0.6 times higher than that of their parents.
芮玉奎1, 黄昆仑1, 2, 王为民3,郭晶1, 金银花1,罗云波1*. 近红外光谱技术在检测转基因油菜籽中芥酸和硫甙上的应用研究[J]. 光谱学与光谱分析, 2006, 26(12): 2190-2192.
RIU Yu-kui1, HUANG Kun-lun1, 2, WANG Wei-min3, GUO Jing1, JIN Yin-hua1, LUO Yun-bo1* . Detection of Erucic Acid and Glucosinolate in Intact Rapeseed by Near-Infrared Diffuse Reflectance Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(12): 2190-2192.
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