Abstract：The albumin is the richest protein in blood circulatory system, which can combine with many drugs and play an important role in transporing protein. In the present work, the non-covalent interaction between human serum albumin and cinnamic acid was studied by using fluorescence quenching method. The results showed that cinnamic acid had a powerful ability to quench the fluorescence of human serum albumin at excitation and emission wavelengths of λex=286 nm and λex=340 nm, respectively, in the reaction solution of pH 7.4. The binding constants(K) at 37 and 47 ℃ were found to be 1.276 7×103 and 3.404 1×103 L·mol-1, with the number of binding site(n) of 0.758 6 and 0.835 6, respectively, suggesting that the reaction temperature is advantageous for the binding reaction in a way. The changes in the thermodynamic parameters of binding interaction at 37 and 47 ℃ indicated that the main binding force between cinnamic acid and human serum albumin was hydrophobic force. These provide important information for studying the pharmacological effects of cinnamic acid and the influence of cinnamic acid on the configuration change of HSA.
赵妍,曹燚,韩凤梅,陈勇*. 荧光猝灭法对肉桂酸与人血清白蛋白间的相互作用的研究[J]. 光谱学与光谱分析, 2008, 28(04): 904-907.
ZHAO Yan,CAO Yi,HAN Feng-mei,CHEN Yong*. Study on the Interaction between Cinnamic Acid and Human Serum Albumin by Fluorescence Quenching Method. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(04): 904-907.
[1] LIU Yong-ming, LI Gui-zhi, SUN Xi-fen(刘永明，李桂芝，孙希芬). Chinese Journal of Analytical Chemistry(分析化学), 2004, 32(5): 615. [2] YAN Zheng-yu，SHAO Xiu-fen，JIANG Xin-min，et al(严拯宇，邵秀芬，姜新民，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006，26(8): 1494. [3] Michael Peschke, et al. J. Am. Soc. Mass Spectrom.，2004，15：1424. [4] ZHU Wei-feng，ZHANG Ya-hong，LIU Hong-ning(朱卫丰，张亚红，刘红宁). Chinese Traditional Patent Medicine(中成药), 2003，25(6): 443. [5] CHEN Xiao-bo，KANG Dong-guo，LI Song, et al(陈晓波，康栋国，李 崧，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006，26(4): 674. [6] LI Bing-yang，TONG Lu，ZOU Ben-tian(李炳阳，童 路，邹本田). Acta Pharmaceutica Sinica(药学学报)，1985，20(5)：398. [7] CHA Dan-ming，LI Shu-ting，YANG Yong-fei，et al(查丹明，李舒婷，杨勇飞，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，1999，19(6)：788. [8] Alain M，Michel B，Michel A. J. Chem. Edu.，1986，63(4)：365. [9] YAN Cheng-nong, TONG Jin-qiang, XIONG Dan, et al(颜承农，童金强，熊 丹，等). Chinese Journal of Analytical Chemistry(分析化学), 2006, 34(6): 796.
