Abstract:A new method for the determination of trace arsenic was developed by using fluorescence resonance energy transfer from acridine orange(AO) to rhodamine B(RB). It was found that under the condition of λex/λem=470/580 nm, effective energy transfer could occur between AO and RB in the dodecyl benzene sodium sulfonate solution. The fluorescence intensity of RB was diminished by molybdoarsenide which was formed by the reaction of arsenic(Ⅴ) with molybdate in sulfuric acid medium. The detection limit of this method was 2.56 μg·L-1. This method was used for the determination of trace arsenic in tea. The range of determination for arsenic was 0.01-0.25 mg·L-1. The relative standard deviation for the determination of arsenic was 0.48%-0.64%. The recoveries for the addition of 0.01-0.03 mg·L-1 arsenic were 98%-103%. The method has been applied to the determination of arsenic with satisfactory results.
Key words:Acridine orange;Rhodamine B;Fluorescence resonance energy transfer;Fluorescence quenching;Arsenic in tea
刘保生,高 静,刘智超,于丽娜,杨大成,杨更亮. 吖啶橙-罗丹明B荧光共振能量转移猝灭法测定砷[J]. 光谱学与光谱分析, 2006, 26(02): 306-308.
LIU Bao-sheng, GAO Jing, LIU Zhi-chao, YU Li-na, YANG Da-cheng, YANG Geng-liang. Fluorescence Resonance Energy Transfer Quenching Method for Determination of Arsenic with Acridine Orange-Rhodamine B. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(02): 306-308.
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