Abstract：Sodium dodecylsulfate(SDS) can greatly enhance the emission intensity of the weak chemiluminescence reaction of Ce(Ⅳ) with norfloxacin(NFLX) in acid medium. Based on this phenomenon, a simple and rapid new method for the continuous determination of norfloxacin was developed. The calibration graph is linear for 7.78×10-8-5.82×10-6 g·mL-1, the detection limit(3σ) is 1.57×10-8 g·mL-1, and the relative standard deviation (n=10, c=1.94×10-6 g·mL-1) is 1.7%. The method was successfully applied to the determination of norfloxacin in capsules. Additionally, the system’s mechanism was discussed.
[1] Ito A, Hirai K, Inoue M, et al. Antimicrob. Agents Chemother., 1980, 17(2): 103. [2] Sultan S M, Suliman F E O. Analyst, 1993, 118: 573. [3] Vílchez J L, Ballesteros O, Taoufiki J, et al. Anal. Chim. Acta, 2001, 444: 279. [4] LI Jian-qing, FENG Xiao-hua, DONG Chuan(李建晴, 冯小花, 董 川). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003, 23(2): 311. [5] Rahman N, Ahmad Y, Hejaz Azmi SN. Eur. J. Pharm. Biopharm., 2004, 57(2): 359. [6] Mascher H J, Kikuta C. J. Chromatogr. A, 1998, 812: 381. [7] Wallis S C, Charles B G, Gahan L R. J. Chromatogr. B, 1995, 674: 306. [8] Rao Y, Tong Y, Zhang X R, et al. Anal. Lett., 2000, 33: 1117. [9] Aly F A, Al-Tamimi S A, Alwarthan A A. Talanta, 2001, 53: 885. [10] LIAN Ning, SUN Chun-yan, ZHAO Hui-chun(连 宁, 孙春燕, 赵慧春). Journal of Analytical Science(分析科学学报), 2002, 18(2): 111. [11] CHEN Guo-nan, ZHANG Fan(陈国南, 张 帆). Chemiluminscence and Bioluminescence-Theory and Application(化学发光与生物发光——理论及应用). Fuzhou: Fujian Science and Technology Press(福州: 福建科技出版社), 1998. 265. [12] Wolkoff A W, Larose R H. Anal. Chem., 1975, 47: 1005.
