Effects of Ultrasound Induction on Genomic DNA of Housefly Larvae
AN Chun-ju1, LI Feng2, ZHANG Li-jin2, LI De-sen1, DU Rong-qian1*
1. College of Life Science, Nankai University, Tianjin 300071, China 2. Department of Applied Chemistry, China Agricultural University, Beijing 100094, China
Abstract：The genomic DNA of housefly larvae was extracted after ultrasound induction, and the structure was analyzed by UV, fluorescence, IR and 1H NMR. The 3′-end of attacin gene was sequenced and compared by means of PCR. All the results indicated that ultrasound induction can destroy the second structure and the base stacking of genomic DNA of housefly larvae, which will result in mismatch repair during DNA duplication and finally change the sequence of DNA, but it has no significant effect on chemical groups and chemical band of genomic DNA.
Key words：Genomic DNA of housefly larvae;Ultrasound induction;DNA structure
安春菊1，李 锋2，张立金2，李德森1，杜荣骞1* . 超声诱导对家蝇幼虫基因组DNA影响的谱学研究[J]. 光谱学与光谱分析, 2005, 25(12): 2020-2023.
AN Chun-ju1, LI Feng2, ZHANG Li-jin2, LI De-sen1, DU Rong-qian1* . Effects of Ultrasound Induction on Genomic DNA of Housefly Larvae. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(12): 2020-2023.
