Abstract：A new method of quantitative determination for serum albumin in aqueous solution has been developed by measuring total internal reflection synchronous fluorescence at the solid/liquid interface. The combination of bovine serum album in (BSA) and meso-tetrakis (4-sulphonatophenyl) porphyrin (TPPS) adsorbed onto the glass surface produced a synchronous fluorescence signal at 421 nm. At pH 4.25, the signal intensity of BSA adsorbed on the interface was proportional to the BSA concentration in bulk solution. The linear range of 1.0-8.0 μg·mL-1 and the detection limit of 0.94 μg·mL-1 were obtained. The human serum samples were determined with satisfactory results.
陈 莹，姚闽娜，唐尧基，李耀群*. 卟啉全内反射同步荧光法测定蛋白质[J]. 光谱学与光谱分析, 2005, 25(12): 2048-2051.
CHEN Ying, YAO Min-na, TANG Yao-ji, LI Yao-qun*. Determination of Protein with TPPS by Total Internal Reflection Synchronous Fluorescence Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(12): 2048-2051.
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