Analysis of Oxygen Hemoglobin in Nasopharyngeal Carcinoma Based on NIR-SERS With Statistical Methods
GAO Fei1*, ZHANG Ming1, KONG De-ying2, ZHANG Jian-ming3
1. Department of Physics，Zunyi Medical University，Zunyi 563000，China
2. Department of Physiology，Zunyi Medical University，Zunyi 563000，China
3. Blood Transfusion Department，The First People's Hospital of Zunyi, Zunyi 563000，China
Abstract：Near-infrared (NIR) Surface Enhancement Raman scattering (SERS) is a highly sensitive and selective surface-matter detection technique that uses oxygenated hemoglobin in human blood to detect the corresponding NIR-SERS spectra. The spectral data were detected and analyzed using a combined multivariate statistical method. Firstly, the NIR-SERS spectra of oxygenated hemoglobin in 30 healthy subjects and 30 patients with nasopharyngeal carcinoma were screened, and it was found that the average NIR-SERS spectra of oxygenated hemoglobin in NPC patients were at the positions of 340, 479, 817, 1 127, 1 215, 1 347, 1 425, 1 587 cm-1. The area and intensity of the peaks were relatively small, especially at the positions of 479, 817 and 1 587 cm-1. In addition, when the NIR-SERS peaks of healthy individuals were at 335, 471, 1 338 and 1 429 cm-1, the red shift to 340, 479, 1 347 cm-1and blue shift to 1425 were observed in NPC patients.The multivariate statistical model of PCA-T (principal component analysis combined with independent variable t-test) was established to obtain the three most significant PCA scores: PC1, PC3, and PC7, two-dimensional and three-dimensional scatter plots were made for the three PCA scores, and the healthy people and NPC patients were divided into different regions. A working characteristic curve (ROC) was used to evaluate the reliability of the PCA-T analysis method and verify its reliability. The results showed that the vibrational changes of oxyhemoglobin molecules, such as pyrrole ring and vinyl group, were abnormal, and the changes of L-Phenylalanine were also significant. The nasopharynx of the human body likely has pathological changes, resulting in the patient's body's more vigorous metabolism of amino acids, increased sugar consumption, and other causes. In conclusion, the combination of near-infraredsurface-enhanced Tunku Abdul Rahman scattering spectroscopy with multivariate statistical analysis has the potential to accurately, effectively, and rapidly differentiate healthy human samples from those of nasopharyngeal carcinoma, this study provides a preliminary experimental basis for the application of NIR-SERS spectroscopy in nasopharyngeal carcinoma and other medical studies and may be a potentially sensitive and reliable tool for early clinical cancer diagnosis.
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