GC-MS and FTIR Analysis and Identification of Moldy Tobacco Leaves
YE Yan-qing1, ZHANG Hai-yu1, 2, SHEN Di1, 2, LE Zhi-wei3, WU Yu-ping4, KONG Guang-hui4, ZHANG Jian-rong2, TIAN Meng-yu2, CHEN Jian-hua2, ZHANG Cheng-ming2*, WANG Jin2*
1. School of Chemistry and Environment, Yunnan Minzu University, Kunming 650504, China
2. Technical Center, Yunnan Tobacco Industry Co., Ltd., Kunming 650106, China
3. Kunming Cigarette Factory，Hongyun Honghe Tobacco (Group) Co., Ltd., Kunming 650231, China
4. Yunnan Tobacco Agricultural Science Research Institute, Kunming 650031，China
Abstract：GC-MS and FTIR analysis, combined with multivariate analysis methods such as PCA and PLS, were used to evaluate and identify moldy tobacco leaves. GC-MS analysis screened 9 markers of tobacco leaf mold, including 2-ethylhexanol. The linear discriminant equation constructed by 9 key compounds, such as 4-hydroxybutyrolactone, can accurately identify tobacco leaf mold, with an initial validation accuracy of 100% and a cross-validation accuracy of 98.7%. FTIR studies have shown that the moldy process of tobacco leaves consumes a large amount of carbohydrates, proteins, and lipids. GC-MS and FTIR, combined with PCA and PLS-DA respectively, can effectively distinguish moldy tobacco leaves.
[1] CHEN Wan-nian, SONG Ji-zhen, FAN Jian-qiang, et al（陈万年, 宋纪真, 范坚强, 等）. Tobacco Technology（烟草科技）, 2003, (7): 9.
[2] QI Ling-feng, ZHUO Si-chu, SONG Ji-zhen, et al（齐凌峰, 卓思楚, 宋纪真, 等）. Anhui Agricultural Science（安徽农业科学）, 2010, 38(1): 155.
[3] QI Ling-feng, ZHUO Si-chu, SONG Ji-zhen, et al（齐凌峰, 卓思楚, 宋纪真, 等）. Hubei Agricultural Science（湖北农业科学）, 2014, 53(1): 106.
[4] CHEN Yue-li, XIAO Ming-li, YANG Qing（陈越立, 肖明礼, 杨 庆）. Guangdong Chemical Industry（广东化工）, 2011, 38(4): 49.
[5] QIU Li-you, ZHAO Ming-qin, YUE Mei, et al（邱立友, 赵铭钦, 岳 梅, 等）. Tobacco Technology（烟草科技）, 2003, (3): 14.
[6] YC/T 475-2013. Tobacco and Tobacco Producs-Molding Control Guid(烟草及烟草制品霉变控制指南). Beijing: China National Tobacco Corproration(北京：中国烟草总公司), 2013.
[7] Zhang H M, Wang J, Tian X J, et al. Journal of Food Engineering, 2007, 82：403.
[8] QI Da-wei, LIN Hua-qing, SHA Yun-fei, et al（戚大伟，林华清，沙云菲，等）. Tobacco Technology(烟草科技)，2013，(5): 5.
[9] Larsen T, Axelsen J, Ravn H W. Journal of Chromatography A, 2004, 1026(1): 301.
[10] Li S P, Lai C M, Gong Y, et al. Journal of Chromatography A, 2004, 1306(2): 239.
[11] Sha Y F, Deng C H, Liu B Z. Journal of Chromatography A, 2008, 1198/1199: 27.
[12] HUANG Long, WANG Jin-yuan, LUO Cheng-hao, et al（黄 龙，王进元，罗诚浩，等）. Tobacco Technology（烟草科技）, 2006, (10): 41.
[13] GUO Le-le, ZHANG Xiao-ping, LIU Fang, et al（郭乐乐，张晓平，刘 芳，等）. Tobacco Technology（烟草科技）, 2020, 53(2): 27.
[14] ZHOU Ji-yue, YANG Pan-pan, LIU Lei, et al（周继月，杨盼盼，刘 磊，等）. Chinese Journal of Tobacco（中国烟草学报），2018，24(1): 6.
[15] CHEN Zhao-lin, ZHANG Yi, WANG Shi-yu，et al（陈兆麟，张 奕，王诗羽，等）. China Computer & Communication（信息与电脑）, 2022，34(22): 118.
[16] Liu K W, Zhang C. Food Chemistry, 2021，334：127615.
[17] Qi J, Zhou Q, Huang D, et al. Microbial Cell Factories, 2023, 22(1)：230.
[18] Oktaviary R, Nugrahani I, Ibrahim S, et al. Indonesian Journal of Pharmacy, 2020, 31(2): 78.
[19] Soares R, Lima A M F, Oliveira R V B, et al. Polymer Degradation & Stability, 2005, 90(3): 449.
[20] Diao Y, Song M, Zhang Y, et al. Carbohydrate Polymers, 2017, 169: 92.
[21] Ke-Chang Hung, Chen Y L, Wu J H. Polymer Degradation and Stability, 2012, 97: 1680.
[22] Sunesson A L S, Vaes W H J, Nilsson C A, et al. Applied and Environmental Microbiology, 1995, 61(8): 2911.
[23] Kleofas V, Popa F, Fraatz M A, et al. Flavour and Fragrance Journal，2015, 30(5)：381.
[24] Benito M J, Núñez F, Córdoba M G, et al. Food Microbiology 2005, 22(6)：513.
[25] Laraba I, McCormick S P, Vaughan M M, et al. Fungal Genet Biol，2020, 144: 103466.
[26] Simonato B, Lorenzini M, Zapparoli G. LWT-Food Science and Technology，2021, 138: 110620.
[27] LópezL, EcheverriaG, Usall J, et al. Postharvest Biology and Technology 2015, 99：120.
[28] Piesik D, Pańka D, Delaney K J, et al. Journal of Plant Physiology，2011, 168(9): 878.
[29] Zhou J, Cheng Y, Yu L, et al. Applied Microbiology and Biotechnology，2022, 106: 131.
[30] Liu K, Zhang C, Xu J, et al. Comprehensive Reviews in Food Science and Food Safety, 2021, 20(6): 5802.
[31] YC/T 159-2002. Tobacco and Tobacco Products-Determination of Water Soluble Sugars-Continuous Flow Method(烟草及烟草制品水溶性糖的测定——连续流动法). Beijing: China National Tobacco Corporation(北京：中国烟草总公司), 2002.
[32] YC/T 249-2008. Tobacco and Tobacco Products-Determination of Protein-Continuous Flow Method(烟草及烟草制品蛋白质的测定——连续流动法). Beijing: China National Tobacco Corporation(北京：中国烟草总公司), 2002.
[33] WU De-zhuan, ZHOU Ji-heng, LI Zhang-hai, et al（武德传，周冀衡，李章海，等）. Chinese Tobacco Science（中国烟草科学）, 2010，31(3): 4.
[34] WU Shun, LOU Hong-ming, MO Xian-ke, et al（吴 舜，楼宏铭，莫贤科，等）. Tobacco Technology（烟草科技），2014，(10): 4.
