Rapid Identification of Sulfamethazine and Sulfadiazine Residues in Chicken Based on SERS
XU Ning1, 2, LIU Mu-hua1, 2, YUAN Hai-chao1, 2, HUANG Shuang-gen1, 2, WANG Xiao1, 2, ZHAO Jin-hui1, 2*, CHEN Jian1, 2, WANG Ting1, 2, HU Wei1, 2, SONG Yi-xin1, 2
1. College of Engineering Optics-Electrics Application of Biomaterials Key Laboratory of Bioelectro-Optics and Applications, Jiangxi Agricultural University, Nanchang 330045, China
2. Collaborative Innovation Center of Postharvest Key Technology and Quality Safety of Fruits and Vegetables in Jiangxi Province, Nanchang 330045, China
Abstract：Surface-enhanced Raman spectroscopy (SERS) of chicken was collected by DXRTM micro-Raman spectrometer with gold colloid as an active substrate and NaCl solution as the active agent. Rapid identification of sulfadimidine (SM-2) and sulfadiazine (SPD) residues in chicken was achieved. Raman peaks at 937 and 1 188 cm-1 were used to determine whether there are SM-2 and SPD in chicken or not. The Single-factor experiment method was used to optimize the experimental conditions, and the optimum experimental conditions were obtained: the addition amount of Gold glue was 500 L, the addition amount of NaCl solutionwas 100 L and the adsorption time was 5 minutes. The original Raman spectra were pre-processed by adaptive iterative penalty least squares (air-PLS), normalization and second derivative. Then the eigenvectors were extracted by principal component analysis (PCA). Finally, the first four PCA scores were used as input values of the support vector machine (SVM) classification model, and the SVM classification model based on C-SVC type was established. The optimal penalty parameter c was 0.01, and the kernel parameter g was 0.1. The overall classification accuracy of the model was 93.23%, the sensitivity of chicken containing SM-2+SPD was 100%, and the specificity of chicken containing SPD was 99.02%. The results showed that this method had good identification effects. It could be used to detect and identify SM-2 and SPD antibiotic residues in chicken quickly.
Key words：Surface-enhanced Raman spectroscopy; Sulfamethazine; Sulfapyridine; Identification; Principal component analysis; Support vector machine
徐 宁，刘木华，袁海超，黄双根，王 晓，赵进辉，陈 健，王 婷，胡 围，宋怡欣. 鸡肉中磺胺二甲嘧啶和磺胺吡啶残留的SERS快速鉴别研究[J]. 光谱学与光谱分析, 2021, 41(03): 924-931.
XU Ning, LIU Mu-hua, YUAN Hai-chao, HUANG Shuang-gen, WANG Xiao, ZHAO Jin-hui, CHEN Jian, WANG Ting, HU Wei, SONG Yi-xin. Rapid Identification of Sulfamethazine and Sulfadiazine Residues in Chicken Based on SERS. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 924-931.
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