Determination of Mineral Elements in Brown Rice of Near-Isogenic Lines Population for Japonica Rice by ICP-AES
ZENG Ya-wen1,3, WANG Lu-xiang2*, SUN Zheng-hai1,4, YANG Shu-ming1, DU Juan1, LI Qi-wan2, PU Xiao-ying1, DU Wei1, XIAO Feng-hui3
1. Biotechnology and Genetic Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China 2. Institute of Quality Standards and Testing Technology, Yunnan Academy of Agricultural Sciences, Kunming 650223, China 3. Yunnan Agricultural University, Kunming 650201, China 4. School of Horticulture and Gardening, Southwest Forestry University, Kunming 650224, China
Abstract：In the present paper, the contents of 17 mineral elements (S, Mo, Ni, Fe, Cr, Na, Al, Cu, P, Sn, Zn, B, Mn, Mg, Ca, Sr and K) in brown rice of 264 plant lines in near-isogenic lines (NILs) population and parents for Japonica rice were determined by ICP-AES technique. The method proved to be simple, rapid, highly sensitive and accurate, and can be used to determine many elements at the same time. Its recovery ratio obtained by standard addition method ranged between 92.3% and 110.27%, and its RSD was lower than 6%. The analytical results showed that NILs for various mineral elements based on the BC5F5 population consisting of 261 lines were developed from a cross between NIL (BC4F5) and Towada. To our knowledge, this is the first report on that near-isogenic lines NIL(BC4F5) with the important value for high mineral elements associated with cold tolerance at booting stage have been bred. The results of the determination show that the brown rice is rich in eleven elements such as P, K, Mg, S, Ca, Zn, Mn, Na, Fe, Cu and Al necessary to human health. There is smaller variation of 5 macroelements (11.15%-16.45%) but larger variation of 12 microelements (16.57%-94.96%) in brown rice from populations. There is significant correlation among 95 from 136 pairs in 17 elements, moreover, especially the correlation (0.531**-0.921**) among 15 pairs of six micro-elements (Mo, Ni, Al, Sn, Cr and Sr) associated with the active components of functional rice is relatively higher than that of 10 pairs (0.175**-0.867**) of 5 macroelements (P, K, Ca, Mg and S), revealing the importance of microelements to catalysing and synthesizing the active components of functional rice. The above results provided reliable data and theory bases for gene location and cloning of controlling elements contents in brown rice, and further revealed the molecular and physiological mechanism of the relation between mineral elements in brown rice and cold tolerance at booting stage in japonica rice from Yunnan Province of China.
曾亚文1,3, 汪禄祥2*, 孙正海1, 4, 杨树明1, 杜娟1, 黎其万2, 普晓英1, 杜威1, 萧凤回3. ICP-AES法测定粳稻近等基因系群体间糙米的矿质元素[J]. 光谱学与光谱分析, 2008, 28(12): 2966-2969.
ZENG Ya-wen1,3, WANG Lu-xiang2*, SUN Zheng-hai1,4, YANG Shu-ming1, DU Juan1, LI Qi-wan2, PU Xiao-ying1, DU Wei1, XIAO Feng-hui3. Determination of Mineral Elements in Brown Rice of Near-Isogenic Lines Population for Japonica Rice by ICP-AES. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(12): 2966-2969.
