Study on the Interaction of Cefazolin and Ceftriaxone with Human Serum Albumin with Multi-Spectroscopic and Docking Methods
WANG Yi-run1, HUANG Fang1, LIU Ying1, 2*
1. College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China
2. Beijing Engineering Research Center of Food Environment and Public Health, Minzu University of China, Beijing 100081, China
Abstract：Cefazolin (CFZ) is the first generation of β-lactam semi-synthetic cephalosporin and which are mainly used to treat Gram positive and negative bacteria. Ceftriaxone (CRO) belongs to the third generation of β-lactam broad-spectrum antibiotic and are particularly useful in treating the disease caused by sensitive bacteria and post-surgery infection prevention. Human serum albumin (HSA) is the most abundant protein in plasma and can bind with a variety of endogenous and exogenous compounds reversibly, which is playing a role in storage and transport. Therefore, detailed investigating the interaction of CFZ and CRO with HSA are very important to understand the pharmacokinetic behavior of the CFZ and CRO. The interaction between CFZ and CRO with HSA were investigated with fluorescence spectroscopy, three-dimensional fluorescence spectroscopy, circular dichroism (CD) and molecular docking and so on under imitated physiological conditions. The results showed that CFZ and CRO could interact with HSA to form complex at 298 and 310 K, which led to quench the intrinsic fluorescence of HSA via static quenching. After correcting the binding parameters, the Stern-Volmer quenching constants (KSV) and binding constants (Ka) of HSA-CFZ and HSA-CRO system inversely correlated with temperatures, and there was one binding site between HSA-CFZ or HSA-CRO system. Based on the Fster’s theory of non-radiation energy transfer, the specific binding distance between HSA-CFZ or HSA-CRO system was 2.41 or 1.40 nm, respectively. Hill’s coefficients (nH<1) proved that a negative cooperativity was found when CFZ or CRO bound to HSA. The thermodynamic parameters (ΔH(HSA-CFZ)=-22.67 kJ·mol-1, ΔH(HSA-CRO)=-39.56 kJ·mol-1, ΔS(HSA-CFZ)=-4.90 J·mol-1·K-1, ΔS(HSA-CRO)=-37.28 J·mol-1·K-1) demonstrated that CFZ and CRO can spontaneously bind with HSA through hydrogen bonds and van der Waals forces. Three-dimensional fluorescence spectroscopy and circular dichroism (CD) showed that CFZ and CRO could change the micro-environment and conformation of HSA. The results of molecular docking revealed that CFZ and CRO were located in Sudlow’s site Ⅰ of HSA proven bycompetitive binding experiments. This study will be helpful to understand the mechanism which affects the conformation and function of HSA with CFZ and CRO in biological processes.
Key words：Cefazolin; Ceftriaxone; Human serum albumin; Multi-Spectroscopy; Molecular docking
王艺润，黄 芳，刘 颖. 多光谱技术结合分子模拟研究头孢唑林和头孢曲松与人血清白蛋白的相互作用[J]. 光谱学与光谱分析, 2017, 37(04): 1205-1210.
WANG Yi-run, HUANG Fang, LIU Ying. Study on the Interaction of Cefazolin and Ceftriaxone with Human Serum Albumin with Multi-Spectroscopic and Docking Methods. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(04): 1205-1210.
[1] Zhang J, Ouyang J B, Zhang H J, et al. Fluid Phase Equilibria，2015, 387: 95.
[2] NI Hong-chang(倪鸿昌). Chinese Journal of Drug Application and Monitoring(中国药物应用与监测), 2009, 6(2): 98.
[3] Dong C Y, Ma S Y, Liu Y. Spectrochim. Acta A，2013, 103: 179.
[4] HU Tao-ying, CHEN Lin, LIU Ying(胡涛英，陈 琳，刘 颖). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2014, 34(5): 1343.
[5] Deng F Y, Liu Y. J. Lumin.，2012, 132(2): 443.
[6] Cao Z Z, Liu R T, Yang B J. Spectrochim. Acta A，2013, 115: 457.
[7] Ross P D, Subramanian S. Biochemistry，1981, 20: 3096.
[8] Xu Q, Lu Y N, Hu X Y, et al. Spectrochim. Acta A，2014, 123: 327.
[9] Hu T Y, Liu Y. J. Pharmaceut. Biomed.，2015, 107: 325.
[10] Bojko B, Sukowska A, Macizek-Jurzyk M, et al. J. Pharmaceut. Biomed.，2010, 52: 383.
[11] Saboury A A. J. Therm. Anal. Calorim., 2003, 72: 93.
