FTIR Spectroscopic Characterization of Material Composition in Functional Leaf of Cotton under Stress of Potassium and Boron
WU Xiu-wen, HAO Yan-shu, LEI Jing, JIANG Cun-cang*
Microelement Research Center, Huazhong Agricultural University, Key Laboratory of Arable Land Conservation Ministry of Agriculture, Wuhan 430070, China
Abstract:Potassium (K) and boron (B) are essential nutrient elements for plants, and the elements play an important role for plant growth, development and physiological metabolism. Cotton has a higher demand for K and B; K deficiency or B deficiency often occurs in cotton though. To reveal the component changes in functional leaf of cotton under K and B stress and investigate effects on material composition from K and B. A pot experiment was conducted at Huazhong Agricultural University. (1) the characteristic peaks at 1 546.86,1 438.85, 1 153.39 and 1 024.17 cm-1 disappeared due to B deficiency, and relative absorbance of other characteristic peaks was decreased compared with normal, which suggested that the structures of protein, fiber, soluble sugar and ribosome in cotton functional leaf changed and decreased in cotent when lack of K. (2) the relative absorbance of all characteristic peaks was increased in the B-deficient cotton leaves compared with normal, suggesting B deficiency leads to the accumulation in leaves of protein, and fiber, soluble sugar and other carbohydrates because of the hindered transportation. (3) lack of both potassium and boron, induced significant changes to both the locations and relative absorbance of characteristic peaks, and the content of protein, and soluble sugar and other carbohydrates increased , while the content of nucleic acids and polysaccharides dropped. K deficiency led to the structures of protein, fiber, soluble sugar and ribosome in cotton functional leaf changed and decreased in content ; B deficiency gave rise to the accumulation in leaves of protein, and fiber, soluble sugar and other carbohydrates; the content of protein and soluble sugar and other carbohydrates increased, while the content of nucleic acids and polysaccharides dropped when K and B were all in short supply .
Key words:Potassium;Boron;Material composition in functional leaf of cotton;FTIR
吴秀文,郝艳淑,雷 晶,姜存仓* . 钾硼胁迫对棉花功能叶物质成分影响的FTIR表征 [J]. 光谱学与光谱分析, 2016, 36(03): 676-680.
WU Xiu-wen, HAO Yan-shu, LEI Jing, JIANG Cun-cang* . FTIR Spectroscopic Characterization of Material Composition in Functional Leaf of Cotton under Stress of Potassium and Boron. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(03): 676-680.
[1] WANG De-peng, TANG Hao-yue, ZHOU Xiao-bin, et al(王德鹏, 唐浩月, 周晓彬, 等). China Cotton(中国棉花), 2011, 38(10): 2. [2] ZHANG Zhi-yong, WANG Qing-lian, LI Zhao-hu, et al(张志勇, 王清连, 李召虎, 等). Acta Agron Sin(作物学报), 2009, 35(4): 718. [3] JIANG Cun-cang, CHEN Fang, GAO Xiang-zhao, et al(姜存仓, 陈 防, 高祥照, 等). Scientia Agricultura Sinica(中国农业科学), 2008, 41(2): 488. [4] Pettigrew W T. Agronomy Journal, 1999, 91(6): 962. [5] Reddy K R, Zhao D L. Field Crops Research, 2005, 94: 201. [6] CAO Yu-lin, TIAN Shao-ren, YANG Shao-qun(操宇琳, 田绍仁, 杨绍群). Cotton Sciences(棉花科学), 2011, 33(6): 58. [7] WANG Chen, JIANG Ze-ping, LI Wen-qing, et al(王 成, 蒋泽平, 李文青, 等). Journal of Agro-Environment Science(中国环境科学学报), 2014, 33(4): 673. [8] Griffiths P, De Haseth J A. Fourier Transform Infrared Spectrometry(2nd ed). New Jersey, USA: John Wiley &Sons, Inc, 2006. [9] WANG Sheng-feng, LIU Yun-xia, HAN Ya, et al(王盛锋, 刘云霞, 韩 亚, 等). Plant Nutrition and Fertilizer Science(植物营养与肥料学报), 2014, 20(4): 1005. [10] Abidi N, Cabrales L, Haigler C H. Carbohydrate Polymers, 2014,(100): 9. [11] XUE Sheng-guo, ZHU Feng, YE Chen, et al(薛生国, 朱 锋, 叶 晨, 等). Acta Ecologica Sinica(生态学报), 2011, 31(20): 6143. [12] McCann M C, Defemez M, Urbanowicz B R, et al. Plant Physiology, 2007, 143: 1314. [13] Liu Guidong, Dong Xiaochang, Liu Leichao, et al. Scientia Horticulturae, 2014, 174: 54. [14] Barron C, Parker M L, Mills E N C, et al. Planta, 2005, 220: 667. [15] XU Rong, LIU You-gang, SUN Su-qin, et al(徐 荣, 刘友刚, 孙素琴, 等). Journal of Nuclear Agricultural Sciences(核农学报), 2009, 23(2): 262. [16] SUN Cai-xia, WU Xiao-fei, WANG Jie, et al(孙彩霞, 吴晓菲, 王 杰, 等). Journal of Northeastern University(东北大学学报), 2012, 33(7): 1060. [17] HU Wen-he, SONG Hong-kai, MA Jin-hua, et al(胡文河, 宋红凯, 马金华, 等). Journal of Jilin Agricultural University(吉林农业大学学报), 201 1, 33(2): 130. [18] CHEN Kun, LIU Shi-qi, ZHANG Zi-kun, et al(陈 昆, 刘世琦, 张自坤, 等). Plant Nutrition and Fertilizer Science(植物营养与肥料学报), 2011, 17(2): 506. [19] Yang J, Yen H E. Plant Physiology, 2002,130: 1032. [20] Camacho-Cristobal J J, Lunar L, Lafont F, et al. Journal of Plant Physiology, 2004, 161: 879. [21] Zhao D, Oosterhuis D M. Field Crop Research, 2002,(78): 75. [22] JIAO Xiao-yan, WANG Jin-song, WU Ai-lian, et al(焦晓燕, 王劲松, 武爱莲, 等). Plant Nutrition and Fertilizer Science(植物营养与肥料学报), 2013, 19(3): 615. [23] LIU Peng, YANG Yu-ai(刘 鹏, 杨玉爱).Plant Nutrition and Fertilizer Science(植物营养与肥料学报), 2003, 9(4): 456. [24] Liu G D, Dong X C, Liu L C, et al. Physiologia Plantarum, 2015, 153(4): 513. [25] Christian Zrb, Mehmet Senbayramb, Edgar Peiterc.Journal of Plant Physiology, 2014, 171: 656.