SERS Spectra of Xanthomonas Oryzae pv. Oryzae (Xoo) on Nano Silver Film Prepared by Electrolysis Method
KANG Yi-pu1, SI Min-zhen1*, LI Qing-yu2, HUANG Qiong3, LIU Ren-ming1
1. Department of Physics and Electron Science, Chuxiong Normal University, Chuxiong 675000, China 2. Fundamental Department of South West Forest College, Kunming 650224, China 3. Faculty of Plant Protection, Yunnan Agricultural University, Kunming 650201, China
Abstract：The nano silver film was prepared by electrolysis method using silver nitrate and polyvinyl alcohol (PVA) in deionized water as the electrolyte, with four glass slides put in the electrolyte and two silver rods dipped into the electrolyte as the anode and cathode. A direct current was applied to the rods, then the four glass slides stayed in the silver colloids. Thus the authors got the nano silver film. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were employed to detect the silver particles in the silver colloids and on the nano silver film. From the SEM we can see that the silver particles on the film formed different layers. In one layer, the distance between two particles was about 100 nm. The samples of Xanthomonas oryzae pv. Oryzae (Xoo) were 7 different kinds of bacterial blight, namely 1-YN1, 2-YN7, 3-YN11, 4-GD414, 5-SCYC6, 6-HEN11 and 7-FWJ. Because the silver particles in the colloids were aggregated on the film, there was large electromagnetic potentiation. So the SERS spectra of Xoo were perfect. The authors used the area analytical method to distinguish the different kinds of Xoo. The silver film prepared by electrolysis was cheap and active, the preparation time of the samples was short, and any normal chemistry lab can make it, which can find excellent application to detecting the Xoo in agriculture. On the other hand, this film is active on biomolecules and bioorganism, which may be a new kind of SERS fundus to explain the creation of the SERS. Further study was under way.
康颐璞1，司民真1*，李清玉2，黄 琼3，刘仁明1 . 水稻白叶枯病菌生理小种在纳米银膜上的表面增强拉曼光谱初探[J]. 光谱学与光谱分析, 2010, 30(02): 372-375.
KANG Yi-pu1, SI Min-zhen1*, LI Qing-yu2, HUANG Qiong3, LIU Ren-ming1 . SERS Spectra of Xanthomonas Oryzae pv. Oryzae (Xoo) on Nano Silver Film Prepared by Electrolysis Method . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(02): 372-375.
[1] Moskovits M. Rev. Mod. Phys., 1985, 57: 783. [2] Xiaofang Z, Yan F, Pengxiang Z. Spectrochimica Acta Part A, 2007, 67: 122. [3] Yantu Z, Zhujun Z, Yonghua S. Journal of Chromatography A, 2006, 1129: 34. [4] Le Ru E C, Blackie E, Meyer M, et al. J. Phys. Chem., 2007, 111: 37. [5] Choi S H, Wang D, Williams J R, et al. Applied Surface Science, 2007, 253: 5411. [6] Yakutik I M, Shevchenko G P, Rakhmanov S K. Colloids and Surface, 2004, 242: 175. [7] Xiaomiao H, Yan F. Colloid and Interface Science, 2007, 316: 19. [8] Ratna T, Richard J C Brown, Martin J T Milton. Journal of Raman Spectrum, 2007, 38: 1469. [9] SI Min-zhen, WU Rong-guo, ZHANG Peng-xiang(司民真, 武荣国, 张鹏翔). Acta Photonica Sinica(光子学报), 1999, 28: 839. [10] Wang J J, Saito Y, Batchelder D N, et al. App. Phy. Lett., 2005, 86: 263111. [11] Qiu T, Wu X L, Shen J C, et al. App. Phy. Lett., 2006, 89: 131914. [12] Chu L Y, Shun Y P, Wang C C, et al. Phytochemistry, 2008, 69: 1989. [13] Cho H J, Park Y J, Noh T H, et al. Microbial Pathogenesis, 2008, 44: 473. [14] Moon W J, Cho J Y, Chae Y K. Protein Expression and Purification, 2008, 10: 1016. [15] XU Zhi-gang, SUN Qi-ming, LIU Feng-quan, et al(许志刚，孙启明，刘凤全，等). Chinese Journal of Rice Science(中国水稻科学)，2004，18(5)：469. [16] JI Guang-hai, XIA Xian-ren, XIAO Lu-ting, et al(姬广海, 夏贤仁, 肖鲁婷, 等). Journal of Yunnan Agricultural University(云南农业大学学报), 2004, 19(5): 541. [17] YU Jing, ZHOU Yong-li, CANG Jing, et al(于 晶，周永力，苍 晶，等). Acta Agronomica Sinica(作物学报)，2006, 32: 1611. [18] LIU Yong, MO Xiao-han, YU Qing, et al(刘 勇，莫笑晗，余 清，等). Acta Phytopathologica Sinica(植物病理学报), 2006, 36(4): 310. [19] SI Min-zhen, FANG Yan, PENG Jia-lin, et al(司民真，方 炎，彭家林，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(5): 948. [20] Ranjith Premasiri W, Moir D T, Ziegler L D. Chemical and Biological Sensing Ⅵ, 2005, 5795: 19. [21] Jarvis R M, Goodacre R. Anal. Chem., 2004, 76: 40. [22] Rothschild K J, Andrew J R, De Grip W J, et al. Science, 1976, 191: 1176. [23] Susi H, Sampugna J, Hampson J W, et al. Biochemistry, 1979, 18: 297.
