Study on the Identification of Ganoderma by Multi-Steps Infrared Macro-Fingerprint Method
CHEN Xiao-kang1, HUANG Dong-lan1,2, SUN Su-qin3*, CAO Jia-jia2, WANG Shao-ling1
1.Department of Chemistry, Shaoguan College，Shaoguan 512005, China 2.Faculty of Chemistry and Light Engineering，Guangdong University of Technology，Guangzhou 510090, China 3.Department of Chemistry, Tsinghua University, Beijing 100084, China
Abstract：Ganoderma lucidum, ganoderma atrum, ganderma tsugae Murr.and ganoderma lipsiense can be discriminated and identified by using multi-steps infrared macro-fingerprint method.The 1D-IR spectra, based on the peaks intensity at 1 153 and 1 078 cm-1, which are the fingerprint characteristic peaks of glucoside compounds, show that the content of glucoside compounds of them was in the order of: ganoderma lucidum＞ganoderma atrum＞ganderma tsugae Murr.＞ganoderma lipsiense.Generally, the second derivative IR spectra can clearly enhance the spectra resolution.In the range of 1 600-1 720 cm-1, the position and sharpness of characteristics peaks were very different, and it’s proved that amino acid peptide compounds of them were different.In the 2D-IR spectra, four of them have the same autopeak at 1 100 cm-1, which is the autopeaks of glucoside, but the number of autopeaks of ganoderma lucidum was 4 and its strongest autopeak was 1 040 cm-1, while 5 autopeaks, 4 autopeaks and 5 autopeaks were for ganoderma atrum, ganderma tsugae Murr.and ganoderma lipsiense respectively, and their strongest autopeaks were 1 040, 1 139, 1 140 and 1 134 cm-1 respectively.The multi-steps infrared maro-fingerprint identification testified that the contents of glucoside compounds and amino acid peptide compounds in these four kinds of ganoderma are different.It’s proved that multi-steps infrared maro-fingerprint method can be used to analyze and distinguish ganoderma lucidum, ganoderma atrum, ganderma tsugae Murr.and ganoderma lipsiense.
陈小康1, 黄冬兰1，2, 孙素琴3*, 曹佳佳2, 王少玲1 . 粤北灵芝的红外光谱宏观三级鉴定研究[J]. 光谱学与光谱分析, 2010, 30(01): 78-82.
CHEN Xiao-kang1, HUANG Dong-lan1,2, SUN Su-qin3*, CAO Jia-jia2, WANG Shao-ling1 . Study on the Identification of Ganoderma by Multi-Steps Infrared Macro-Fingerprint Method. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(01): 78-82.
[1] LIN Zhi-bin(林志彬).Modern Research of Ganoderma(灵芝的现代研究).Beijing: Beijing Medical University Press(北京: 北京医科大学出版社), 2001. [2] Pharmacopoeia Committee of Ministry of Health, the People’s Republic of China(中华人民共和国药典委员会编).Pharmacopeia of the People’s Republic of China(中华人民共和国药典).(Vol.Ⅰ).Beijing: Chemical Industry Press(北京: 化学工业出版社), 2005. [3] ZHANG Jie, JIANG Da-cheng(张 洁, 姜大成).Journal of Changchun University of Traditional Chinese Medicine(长春中医学院学报), 2005, 21(10): 62. [4] XING Zeng-tao, YU Qiong-hua, ZHANG Jin-song, et al(邢增涛, 郁琼花, 张劲松, 等).Journal of Chinese Medicinal Materials(中药材), 2004, 27(8): 575. [5] QIAN Yi-fan, ZHANG Lei, PU Cun-hai(钱一帆, 张 磊, 濮存海).Journal of Chinese Medicinal Materials(中药材), 2007, 30(8): 1027. [6] BAI Yan, SUN Su-qin, LI Jun, et al(白 雁，孙素琴，李 军，等).Chinese Traditional Patent Medicine(中成药), 2005, 27(5): 544. [7] WANG Feng-ling, ZHOU Jian-ke, WU Jing, et al(王凤岭, 周建科, 吴 婧, 等).Modern Instruments(现代仪器), 2006, 12: 18. [8] PEI Li-kuan, GUO Bao-lin, SUN Su-qin(裴利宽, 郭宝林, 孙素琴).Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2008, 28(1): 55. [9] Lu G H, Zhou Q, Sun S Q, et al.Journal of Molecular Structure, 2008, 91: 883. [10] BAI Yan, SUN Su-qin, FAN Ke-feng, et al(白 雁, 孙素琴, 樊克锋, 等).Chinese Traditional and Herbal Drugs(中草药), 2006, 37(11): 1661.
