1. Institute of Grassland Science, China Agricultural University, Beijing Major Laboratory, Beijing 100094, China 2. Gansu Branch of Chengdu Daye International Investment Co. Ltd., Jiuquan 735009, China
Abstract：The present research was attempted to predict the qualities of stem of alfalfa (Medicago sativa L.) without separation from the whole plant by near infrared reflectance spectroscopy and discussed the feasibility of using the near infrared reflectance spectra information of the whole object to predict the qualities of a certain part. Sixty six whole alfalfa hay samples of separated stems from leaves were collected and they were distinguishing by years, cultivars, cuts and growing periods. There were 138 calibration samples and 60 validation samplers. Fourier transform- near infrared reflectance spectroscopy(FT-NIRS) and partial least square(PLS) were used to set up the calibration models of stem’s crude protein (CP), neutral detergent fiber(NDF), acid detergent fiber (ADF), crude ash (CA) and in vitro digestible dry matter (IVDDM) contents. All models showed great calibration and prediction performances except the one of stem’s NDF content. The correlation coefficients of cross-validation (rCV) were between 0.852 3 and 0.900 7, the root mean square errors of cross-validation (RMSECV) were between 0.72% and 3.96% and the correlation coefficients of NIRS values and chemical values (r) were between 0.925 5 and 0.951 2. However, rCV, RMSECV and r of the model of stem’s NDF content were 0.821 4, 3.70% and 0.902 0, respectively. It wasn’t exact enough and would be used for rough predicting only. All of the results showed that near infrared reflectance spectra information of whole alfalfa hay could be used to predict some components of its stem exactly. It was the maiden attempt of using near infrared reflectance spectra information of the whole objects to evaluated the qualities of a certain part.
Key words：Alfalfa;Near-infrared reflectance spectroscopy;Separating stems from leaves;Nutrition qualities
齐晓1,韩建国1*, 聂志东1,刘富渊2,张泽宏2,李曼莉1. 利用近红外漫反射光谱法预测紫花苜蓿茎组分营养价值的研究[J]. 光谱学与光谱分析, 2008, 28(09): 2062-2066.
QI Xiao1,HAN Jian-guo1*,NIE Zhi-dong1,LIU Fu-yuan2,ZHANG Ze-hong2,LI Man-li1. Research on Predicting the Qualities of Stem of Alfalfa Hay by Near Infrared Reflectance Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(09): 2062-2066.
[1] Koegel R G, Straub R J. Value-Added Products from Fractionated Alfalfa. Meeting Abstract Accepted by Agricultural Research Services, United States Department of Agriculture, 2000. [2] Sheaffer C C, Martin N P, Lamb J A F S, et al. Agronomy Journal, 2000, 92(4): 733. [3] ZHAO Huan-huan, YAN Yan-lu（赵环环, 严衍禄）. Journal of Maize Sciences（玉米科学）, 1999, 7(3): 77. [4] WEI Liang-ming, YAN Yan-lu, DAI Jing-rui（魏良明, 严衍禄, 戴景瑞）. Scientia Agricultura Sinica（中国农业科学）, 2004, 37(5): 630. [5] Norris K H, Barnes R F, Moore J E, et al. Journal of Animal Science, 1976, 43(4): 889. [6] Garcia-Criado B, Garcia-Ciudad A. Journal of the Science of Food and Agriculture, 1990, 50(4): 479. [7] Berardo N, Odoardi M, Scotton M, et al Ziliotto U. Rivistadi Agronomia, 1996, 30(3): 288. [8] Villamarin B, Fernandez E, Mendez J. Journal of AOAC International, 2002, 85(3): 541. [9] Gislum R, Micklander E, Nielsen J P. Field Crops Research, 2004, 88: 269. [10] BAI Qi-lin, CHEN Shao-jiang, DONG Xiao-ling, et al（白琪林, 陈绍江, 董晓玲, 等）. Spectroscopy and Spectral Analysis（光谱学与光谱分析）, 2004, 24(11): 1345. [11] LIU Qiang, MENG Qing-xiang, BAI Qi-lin, et al（刘 强, 孟庆翔, 白琪林, 等）. Chinese Journal of Animal Science（中国畜牧杂志）, 2005, 41(11): 39. [12] Vazquez de Aldana B R, Garcia-Criado B, Garcia-Ciudad A, et al. Communications in Soil Science and Plant Analysis, 1996, 27: 795. [13] Clark D H, Cary E E, Mayland H F. Agronomy Journal, 1989, 81(1): 91. [14] Cozzolino D, Fassio A, Giminez A. Journal of the Science of Food and Agriculture, 2001, 81(1): 142. [15] Valdes E V, Young L G, McMillan I, et al Winch J E. Canadian Journal of Animal Science, 1985, 65(3): 753. [16] LIU Li-ying, CHEN Hong-zhang（刘丽英, 陈洪章）. Spectroscopy and Spectral Analysis（光谱学与光谱分析）, 2007, 27(2): 275. [17] BAI Qi-lin, CHEN Shao-jiang, DONG Xiao-ling, et al（白琪林, 陈绍江, 董晓玲, 等）. Spectroscopy and Spectral Analysis（光谱学与光谱分析）, 2006, 26(2): 271. [18] ZHANG Li-ying（张丽英）. Feed Analysis and the Determining Technology of Feed Quality, Second Edition（饲料分析及饲料质量检测技术, 第2版). Beijing: China Agricultural University Press（北京: 中国农业大学出版社）, 2003. 46. [19] Van Soest P J. Journal of Animal Science, 1967, 26: 119. [20] Tilley J M A, Terry R A. Journal of the British Grassland Society, 1963, 18: 104.
