Spectroscopic Studies on Interaction of Bovine Hemoglobin and Realgar Nanoparticles
WEI Jing,SHEN Xing-can,LIANG Hong*,LIANG Yu-ning
College of Chemistry and Chemical Engineering, Key Laboratory of Medicinal Chemical Resources and Molecular Engineering, Guangxi Normal University, Guilin 541004, China
Abstract：In the present paper, the interaction of bovine hemoglobin (BHb) and realgar nanoparticles has been investigated by ultraviolet-visible (UV-Vis) and fluorescence spectroscopy. The Soret band of oxygen-BHb at 406 nm shifted to 413 nm, and its absorption intensity decreased gradually after adding realgar nanoparticles. The Soret band decreases gradually with the increasing the amount of realgar NPs, suggesting the detachment of some heme chromophores from their matrixes in BHb. The red-shift of characteristic peak leads to be conjecture that the arsenic of realgar conbined with the oxygen of BHb. The oxygen-BHb was deoxidated by realgar nanoparticles, and the surface binding induces conformation change of BHb from the high-affinity R state to the low-affinity T state. The fluorescence intensity of BHb is quenched by realgar nanoparticles when its concentration gradually increased. The analysis of Stern-Volmer equation revealed that the mechanism was a static quenching procedure. The order of the magnitude of binding constant k was 109, obtained from the calculation of UV-Vis and fluorescence spectra.
[1] Lu D P, Wang Q. International Journal of Hematology, 2002, 76: 316. [2] Ye H Q, Gan L, Yang X L, et al. Journal of Ethnopharmacology, 2006, 103: 366. [3] Deng Y, Xu H, Huang K, et al. Pharmacological Research, 2001, 44: 513. [4] Zhu, Y C, Cheng G J, Dong S J. Biophys. Chem., 2002, 97: 129. [5] Cheng Y, Lin H K, Xue D P, et al. Biochim. Biophys. Acta 2001, 1535: 200. [6] Chen H Y, Long Y T. Anal. Chim. Acta, 1999, 382: 171. [7] Cheng Y, Li Y, Li R C, et al. Chemico-Biological Interactions, 2000, 125: 191. [8] Sil S, Kar M, Chakraborti A S. J. Photochem. Photobio. B: Biology, 1997, 41: 67. [9] CEN Shan, ZHANG Ren, YAO Wen-hua, et al(岑 剡, 张 人, 姚文华, 等). Spectroscopy and Spectral Analysi(光谱学与光谱分析), 2005, 25(3): 405. [10] RUAN Ping, HUANG Yao-xiong, LI Dan(阮 萍, 黄耀熊, 李 丹). Spectroscopy and Spectral Analysi(光谱学与光谱分析), 2005, 25(7): 1121. [11] SHEN Xing-can, LIU Xin-yan, LIANG Hong, et al(沈星灿，刘新艳，梁 宏, 等). Acta Chimica Sinica(化学学报), 2006, 64(6): 469. [12] LIU H H, TIAN Z Q, LU Z X, et al. Biosen. Bioelectr., 2004, 20: 295. [13] NIU Qiu-ya, CHEN Wei-ping, TIAN Yi-zhuang, et al(牛秋雅, 陈维平, 田一庄, 等). Environmental Protection of Chemical Industry(化工环保), 2002, 22(3): 169. [14] SHEN Xing-can, LIANG Hong, CHEN Yun, et al(沈星灿, 梁 宏, 陈 韵, 等). China Patent, 200510020488.5, 2005. [15] Karen T, Leon S. Anal. Chem., 1996, 68: 1755. [16] Faulkner K M, Bonaventura C, Crumbliss A L. Inorg. Chim. Acta, 1994, 226: 187. [17] ZUO Zhi-Ying, BO Yun-hong, YANG Xiao-da, et al(左智颖, 薄云红, 杨晓达, 等). Joural of Rare Earths(中国稀土学报), 1996, 14(4): 336. [18] CHANG Xi-jun, HUANG Yan, HE Qun(常希俊, 黄 艳, 贺 群). Acta Chimica Sinica(化学学报), 2005, 63(3): 222.
