防己诺林碱与牛血清白蛋白相互作用的研究

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摘要：在不同温度下，用荧光猝灭光谱、同步荧光光谱和紫外-可见吸收光谱，研究了防己诺林碱与BSA相互作用的光谱学行为。防己诺林碱对BSA有较强的荧光猝灭作用。根据不同温度下防己诺林碱对BSA的荧光猝灭作用，利用Stern-Volmer方程处理实验数据，表明防己诺林碱对BSA的荧光猝灭作用属于静态猝灭。根据Frster非辐射能量转移理论计算出了防己诺林碱与BSA间的结合距离R(27 ℃ 2.51 nm; 37 ℃ 2.72 nm; 47℃ 2.89 nm)、结合常数K_A(27 ℃ 1.05×10⁵ L·mol^-1; 37 ℃ 3.31×10⁵ L·mol^-1; 47 ℃ 7.24×10⁵ L·mol^-1)及对应温度下的热力学参数。热力学数据表明二者主要靠疏水作用力结合，同时用同步荧光光谱探讨了防己诺林碱对BSA构象的影响。

关键词：防己诺林碱;BSA;荧光猝灭光谱;同步荧光光谱;紫外-可见吸收光谱

Abstract：The binding reaction of fangchinoline with bovine serum albumin was studied at different temperatures by fluorescence quenching spectra, synchronous fluorescence spectra and ultra-violet spectra. It was shown that fangchinoline has a strong ability of quenching the fluorescence of BSA. The Stern-Volmer curve of the fluorescence quenching of BSA by fangchinoline indicated that the quenching mechanism of fangchinoline to BSA was a static quenching. According to the Frster theory of non-radiation energy transfer, the binding distances (r) at different temperature were 2.51 nm (27 ℃), 2.72 nm (37 ℃) and 2.89 nm (47 ℃), respectively, while the binding constants (K_A) were 1.05×10⁵ L·mol^-1 (27 ℃), 3.31×10⁵ L·mol^-1 (37 ℃), and 7.24×10⁵ L·mol^-1 (47 ℃), respectively. The thermodynamic parameters showed that the interaction of fangchinoline and BSA was mainly driven by hydrophobic force. Synchronous spectrum was used to investigate the conformational changes of BSA.

Key words：Fangchinoline;Bovine serum albumin;Fluorescence quenching spectra;Synchronous fluorescence spectra;Ultra-violet spectra

收稿日期: 2006-07-30 修订日期: 2006-11-02

中图分类号:

O641.3

通讯作者: 王志 E-mail: wangzhi@hebau.edu.cn

引用本文:

吴秋华,王春,王志^*,马晶军,臧晓欢,秦娜欣. 防己诺林碱与牛血清白蛋白相互作用的研究[J]. 光谱学与光谱分析, 2007, 27(12): 2498-2501.
WU Qiu-hua,WANG Chun,WANG Zhi^*,MA Jing-jun,ZANG Xiao-huan,QIN Na-xin. Study on the Interaction between Fangchinoline and Bovine Serum Albumin. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(12): 2498-2501.

链接本文:

https://www.gpxygpfx.com/CN/Y2007/V27/I12/2498

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