Abstract：The thiaxanthon-2-yl-oxyacetate was synthesized,and its structure was characterized by elemental analysis,IR,1H NMR,and 13C NMR spectra. It was found from the experiment that thiaxanthon-2-yl-oxyacetate had very intense fluorescence,so spectroscopy methods were used to study the interactions of thiaxanthon-2-yl-oxyacetate and ct-DNA. The ultraviolet absorption intensity of thiaxanthon-2-yl-oxyacetate decreased as the concentration of ct-DNA increased at pH 7.38. There was some debased intensity both in the negative and positive spectra of CD spectra of ct-DNA when thiaxanthon-2-yl-oxyacetate interacted with ct-DNA,and quenching effect occurred in the fluorescence spectrum of thiaxanthon-2-yl-oxyacetate. The quenching constant is 1.25×104 L·mol-1,when the concentration of ct-DNA changed from 0 to 12.0 mg·L-1,and the connection between the quenching data and the concentration of ct-DNA shows a favorable linear relationship. The detection limit was 1.63 mg·L-1,so the compound is a new reagent for the measurement of ct-DNA. The interaction of TYO and ct-DNA was insetted and static.
申少斌,郑秀荣,宋玉民*,卢小林. O-(硫杂蒽酮-[2]-基)-氧乙酸的合成、表征及与DNA作用的研究[J]. 光谱学与光谱分析, 2007, 27(08): 1580-1583.
SHEN Shao-bin,ZHENG Xiu-rong,SONG Yu-min*,LU Xiao-lin. Synthesis and Characterization of Thiaxanthon-2-yl-oxyacetate and Its Combination with ct-DNA. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(08): 1580-1583.
