1. College of Chemical Engineering and Material Science,Zhejiang University of Technology,Hangzhou 310032,China 2. Ningbo Entry-Exit Inspection and Quarantine Bureau of the People’s Republic of China,Ningbo 315012,China
Abstract：In the present paper we report quantitative analysis of glucose using internal standard laser Raman spectra. A good linear correlation was observed between the intensities of the —COO band at 1 125 cm-1 using excition wavelength of 632.81 nm(r=0.998 8) and the glucose concentration over the range 0-1.8 mol·L-1. Band intensities were normalized against an internal standard (water band at 1 643 cm-1),and the limit of detection (L.O.D.) of glucose is 0.022 7 mol·L-1. The interference ions would not influence the quantitative analysis. When this method was used to determine 5% glucose NaCl，5% glucose,and 10% glucose injections,the result showed that the recoveries are 71.88%-126.31%,81.02%-124.89% and 74.87%-121.32%,and the RSDs are 5.44%,4.34% and 0.94%,respectively. The non destructive,non intrusive nature of the method makes internal standard laser Raman spectra a convenient,accurate,and green quantitative analysis method.
Key words：Laser Raman spectra;Glucose;Quantitative determination;Internal standard
