Spectroscopic Studies on the Interactions of a Hexadeca-Carboxy Zinc Phthalocyanine and Serum Albumin and Preparation of the Bioconjugates
CHEN Yan-mei1, HUANG Jian-dong1*, LIU Feng-ran1, SUN Rui-qing1, Dennis K P Ng2
1. College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, China 2. Department of Chemistry, The Chinese University of Hong Kong, Hong Kong, China
Abstract：The interactions of the new photosensitizer {2,3,9,10,16,17,23,24-octakis[(3,5-dicarboxy)phenoxy]-phthalocyaninato}zinc(Ⅱ) [ZnPc(COOH)16] and serum albumins (BSA and HSA) were investigated by absorption and fluorescence spectroscopy. The binding constants were found to be 2.25-2.94×106 L·mol-1. The 1∶1 ZnPc(COOH)16-BSA and ZnPc(COOH)16-BSA conjugates were also prepared by incubating a mixture of ZnPc(COOH)16 and the corresponding serum albumin in 2∶1 mole ratio followed by separation by gel permeation chromatography. The Q band absorption and fluorescence emission of ZnPc(COOH)16 are slightly red-shifted in the conjugates, and the spectral features suggest that the phthalocyanine exists predominantly in monomeric form in the conjugates.
陈燕梅1,黄剑东1*,刘丰冉1,孙瑞卿1,吴基培2 . 十六羧酸基酞菁锌光敏剂与白蛋白相互作用的光谱研究及复合物的制备[J]. 光谱学与光谱分析, 2006, 26(08): 1387-1391.
CHEN Yan-mei1, HUANG Jian-dong1*, LIU Feng-ran1, SUN Rui-qing1, Dennis K P Ng2 . Spectroscopic Studies on the Interactions of a Hexadeca-Carboxy Zinc Phthalocyanine and Serum Albumin and Preparation of the Bioconjugates . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(08): 1387-1391.
