Abstract:The Raman spectra of DNA in different levels of vitamin C with 10 min ultraviolet radiation and 30 min ultraviolet radiation were reported. The intensity of ultraviolet radiation was 18.68 W·m-2. The experimental results proved that vitamin C can reduce DNA damage solely, but the effects are related to the concentration of vitamin C. When the concentration of vitamin C was lower than 0.35 mmol·L-1, DNA was protected better with the rising of vitamin C concentration during ultraviolet radiation. DNA was protected best when vitamin C was 0.35 mmol·L-1. When vitamin C concentration was higher than 0.35 mmol·L-1, vitamin C accelerated DNA damage with 10 min ultraviolet radiation. But DNA was also protected with 30 min ultraviolet radiation as vitamin C was 0.35-0.65 mmol·L-1. However, DNA was only protected slightly at high concentration of vitamin C.
周殿凤,柯惟中*,陈年凤,籍 康. 不同浓度维生素C对DNA紫外损伤的影响[J]. 光谱学与光谱分析, 2005, 25(12): 2012-2015.
ZHOU Dian-feng, KE Wei-zhong*, CHEN Nian-feng, JI Kang . Effects of Different Levels of Vitamin C on UV Radiation-Induced DNA Damage . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(12): 2012-2015.