Spectral Study on the Interaction Mechanism between Thionine and Calf Thymus DNA
DU Jiang-yan1, HUANG Xiao-hua2, XU Fei2, FENG Yu-ying2, XING Wei1, LU Tian-hong1, 2*
1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China 2. Department of Chemistry and Environmental Science, Nanjing Normal University, Nanjing 210097, China
Abstract：The interaction mechanism of thionine (TH) and calf thymus DNA (CT-DNA) was studied with UV-Vis absorption spectroscopy, fluorescence spectroscopy, circular dichroism (CD), and X-ray photoelectron spectroscopy (XPS). It was demonstrated that in the pH 7.2 phosphate buffer solution, the interaction of TH and CT-DNA is in the intercalative mode. The absorbance of the absorption peak of TH decreases and the peak position shifts to the red direction. The binding constant (K) of TH with CT-DNA is 1.45×104 mol·L-1. The fluorescence intensities of TH decrease drastically with increasing the concentration of CT-DNA. The Stern-Volmer quenching constant (KSV) was calculated to be 1.01×104 mol·L-1. The intercalative sites are mainly located at the G-C sequences of the CT-DNA molecule through the S atom in the TH molecule according to the experimental data obtained from the fluorescence spectra and XPS analysis. The secondary structure conformation of CT-DNA is changed due to its interaction with TH.
杜江燕1,2，黄晓华2，徐 飞2，冯玉英2，邢 巍1，陆天虹1, 2* . 硫堇与DNA分子作用机理的光谱研究[J]. 光谱学与光谱分析, 2005, 25(09): 1435-1438.
DU Jiang-yan1, HUANG Xiao-hua2, XU Fei2, FENG Yu-ying2, XING Wei1, LU Tian-hong1, 2* . Spectral Study on the Interaction Mechanism between Thionine and Calf Thymus DNA . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(09): 1435-1438.
[1] Mudasir K, Wijaya N, Yoshioka H I. J. Inorg. Biochem., 2003, 94: 263. [2] Wang Z X, Liu D J, Dong S J. Biophysical Chem., 2000, 87: 179. [3] Mahadevan S, Palaniandavar M. Inorg. Chem., 1998, 37: 3927. [4] HE Ji-xiang, JIANG Chong-qiu, GAO Ming-xia(贺吉香, 江崇球, 高明霞). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003, 23(4): 755. [5] SHI Qiao-cui, PENG Tu-zhi, WANG Su-fen(时巧翠, 彭图治, 王素芬). Chinese J. Anal. Chem.(分析化学), 2003, 31(10): 1212. [6] SHEN Jing-shan, SUN Dan-dan, FU Lian-chun, et al(沈景山，孙丹丹，付连春，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005，25(2)：232. [7] ZHANG Li-he, ZHUO Ji-cang, MA Ling-tai, et al(张礼和, 卓济苍, 马灵台，等). Study on the Mechanism of the Drugs Aimed at DNA(以核酸为作用靶的药物研究). Beijing: Science Press(北京: 科学出版社), 1997. 11. [8] CHENG Gui-fang, ZHANG Dong-mei, DING Min, et al(程圭芳, 张冬梅, 丁 敏，等). Chem. J. Chinese Universities(高等学校化学学报), 2003, 24(8): 1395. [9] Stefania M, Rosanna M, Enzio R. J. Chem. Soc., Perkin Trans., 1998, 2: 1983. [10] YANG Zhou-sheng, YU Jun-sheng, CHEN Hong-yuan(杨周生, 于俊生, 陈洪渊). Chem. J. Chinese Universities(高等学校化学学报), 2002, 23(8): 1457. [11] Joseph W, Mehmet O, Xiaohua C. Bioelectrochem. Bioenerg., 1998, 45: 33. [12] XI Xiao-li, YANG Man-man, HAN Xiao-jian, YANG Pin(席小莉, 杨曼曼, 韩小见, 杨 频). Chinese J. Inorg. Chem.(无机化学学报), 2001, 17(6): 781. [13] Koji H, Keiko I, Yoshio I. Anal. Chem. Acta, 1994: 286. [14] Kumar C V, Emma H A. J. Am. Chem. Soc., 1993, 115(19): 8547. [15] Long E C, Barton J K. Acc. Chem. Res., 1990, 23: 271. [16] Claus A M, Seidel A S, Markus H M. J. Phys. Chem., 1996, 100: 5541. [17] Reid G D, Douglas J W, Mark A D. J. Am. Chem. Soc., 2001, 123(28): 6953. [18] Zhang Z L, Huang W M, Tang J L, et al. Biophysical. Chem., 2002, 97: 7. [19] Yang R, Ruan C M, Dai W L, et al. Electrochimica Acta, 1999, 44: 1585.
