Abstract:The present paper reports photocatalytic degradation of bovine serum albumin (BSA) by TiO2 under near UV(365 nm) irradiation.Ultraviolet-Visible spectrometry, FTIR spectrometry and HPLC were used to study the degradation and characterize the final degradation products.It was found that under near UV irradiation, BSA (0.1 g·L-1) can be totally degraded to small fragments by TiO2 in 5 h.Total mineralization of BSA (93.5%) is reached after reacting for 40 h.C, N and S in BSA are mineralized to give CO2, NO-3 and SO2-4.It is suggested that the degradation of BSA happens in two steps: first to small fragments, then further mineralized to small inorganic molecule.The absence of UV irradiation or TiO2 can only lead to partial degradation of BSA (30%) in 5 h and no CO2 is observed even in longer reaction time.
Key words:Titanium dioxide;Photocatalysis;Bovine serum albumin
杨桂芹,李朝晖*,林章祥,王绪绪,刘 平,付贤智* . TiO2对牛血清白蛋白的光催化降解的研究[J]. 光谱学与光谱分析, 2005, 25(08): 1309-1311.
YANG Gui-qin, LI Zhao-hui*,LIN Zhang-xiang, WANG Xu-xu, LIU Ping,FU Xian-zhi* . Investigation of TiO2 Photocatalytic Degradation of Bovine Serum Albumin. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(08): 1309-1311.
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