Study on the Interaction Mechanism of Ellagic Acid and Urolisine A~D With HSA Based on Spectral Analysis
XIE Xing1, CHENG Xin-peng1, ZHANG Lu1*, LUO Jing1, WANG Le-huai1, LIN Wen-jing1, LU Fei-yan1, TU Zong-cai1, 2*
1. Department of Food Nutrition and Health, College of Health, Jiangxi Normal University, Nanchang 330022, China
2. State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China
Abstract：Spectroscopy and molecular simulation technologies investigated The interaction mechanism between ellagic acid (EA) and urolithin A~D (UA~D) with HSA, which helped explore its pharmacotoxicity and efficacy. The results indicated that EA and UA~D could bind with HSA at a molar ratio of 1∶1 and quench its fluorescence via a static mechanism. The binding of UA and UC with HSA was exothermic and driven by hydrogen bonding and van der Waals force. The binding of EA and UD with HSA was endothermic and driven by hydrophobic interaction. The three-dimensional fluorescence spectrum analysis exhibited that the addition of EA and UC, UD, UA, and UB enhanced the hydrophilicity and hydrophobicity of tyrosine and tryptophan microenvironments of HSA, respectively. The molecular simulation analysis showed that EA and UA~D formed hydrogen bonds with active amino acid residues Lys436, Asp187, Lys432, Arg485, Leu430, Leu4, Ile388 and Tyr411, and formed hydrophobic interaction with active amino acid residues Ala191, Val456, Lys199 and Trp214, which proved that they were majorly bound to HSA by hydrogen bonds and van der Waalsforce, and then screened glycation sites and inhibited HSA glycation. This study could provide a theoretical basis for developing EA and UA~D as non-enzyme glycation inhibitors to treat diabetic complications.
Key words：Human serum albumin; Glycation; Interaction mechanism; Spectral analysis; Ellagitannin and its metabolites
谢 星，程鑫鹏，张 露，罗 晶，王乐怀，林文静，卢菲艳，涂宗财. 基于光谱分析的鞣花酸和尿石素A~D与HSA相互作用机理研究[J]. 光谱学与光谱分析, 2025, 45(01): 282-290.
XIE Xing, CHENG Xin-peng, ZHANG Lu, LUO Jing, WANG Le-huai, LIN Wen-jing, LU Fei-yan, TU Zong-cai. Study on the Interaction Mechanism of Ellagic Acid and Urolisine A~D With HSA Based on Spectral Analysis. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2025, 45(01): 282-290.
[1] Qiu H Y, Hou N N, Shi J F, et al. World Journal of Diabetes, 2021, 12(7): 1057.
[2] Alam M M, Ahmad I, Naseem I. International Journal of Biological Macromolecules, 2015, 79: 336.
[3] Xing X, Chun C, Xiong F, et al. Food & Function, 2021, 12(19): 9315.
[4] Jud P, Sourij H. Diabetes Research and Clinical Practice, 2019, 148: 54.
[5] Sarmah S, Goswami A, Belwal V K, et al. Food Research International, 2022, 157: 111358.
[6] Lipinska L, Klewicka E, Sojka M. Acta Scientiarum Polonorum Technologia Alimentaria, 2014, 13(3): 289.
[7] Tomas-Barberan F A, Gonzalez-Sarrias A, Garcia-Villalba R, et al. Molecular Nutrition & Food Research, 2017, 61(1): 1500901.
[8] Lopez-Yerena A, Perez M, Vallverdu-Queralt A, et al. Pharmaceutics, 2020, 12(11): 1123.
[9] Zhou Z, Hu X, Hong X, et al. Journal of Molecular Liquids, 2020, 297: 111835.
[10] Qais F A, Sarwar T, Ahmad I, et al. International Journal of Biological Macromolecules, 2021, 169: 143.
[11] Zhang D, Zhang X, Liu Y C, et al. Journal of Molecular Liquids, 2018, 258: 155.
[12] Abdullah S M S, Fatma S, Rabbani G, et al. Journal of Molecular Structure, 2017, 1127: 283.
[13] Liu D, Cao X, Kong Y, et al. International Journal of Biological Macromolecules, 2021, 166: 259.
[14] Feroz S R, Mohamad S B, Bujang N, et al. Journal of Agricultural and Food Chemistry, 2012, 60(23): 5899.
[15] Zeng L, Ding H, Hu X, et al. Food Chemistry, 2019, 271: 70.
[16] Wang J, Dadmohammadi Y, Jaiswal A, et al. Journal of Agricultural and Food Chemistry, 2020, 68(37): 10184.
[17] Rabbani G, Lee E J, Ahmad K, et al. Molecular Pharmaceutics, 2018, 15(4): 1445.
[18] Suo Z L, Sun Q M, Yang H Q, et al. RSC Advances, 2018, 8(9): 4742.
[19] Tayyab S, Sam S E, Kabir M Z, et al. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2019, 214: 199.
[20] Razzak M A, Choi S S. Food Chemistry, 2021, 347: 128981.
[21] Zhang L, Zhou W N, Tu Z C, et al. Journal of Agricultural and Food Chemistry, 2020, 68(31): 8263.
[22] Ma J, Yang B, Hu X, et al. Environmental Pollution, 2023, 324: 121342.
