Abstract:Based on the Attenuated Total internal Reflectance Fourier Transform Infrared spectroscopy (ATR-FTIR) to study the main differencesof chemical constituents in water extraction of fresh Radix Rehmanniae (RR), a decoction of crude RR and processed RR. In addition, ion chromatography was used to analyze the different components quantitatively. This study aims to reveal the whole composition and variation of fresh RR and its processed products. In ATR-FTIR, there were differences among the three RR in the region of saccharides. The characteristic peaks of fresh RR were located at 1 140, 1 047 and 1 000 cm-1, raw RR were 1 140 and 1 045 cm-1, and processed RR were 1 142 and 1 029 cm-1. Moreover, the peak shapes of the three kinds of RR were quite different. Meanwhile, the second derivative infrared spectra (SDIR) showed that the characteristic peaks of saccharides in the range of 1 200~600 cm-1 changed most significantly during the processing of RR. The positions of characteristic peaks of three kinds of RR extracts were similar, but the relative peak intensities were different, and they changed regularly with the processing process. It can be inferred that during the processing of fresh RR, the polysaccharides in fresh RR hydrolyzed into oligosaccharides or monosaccharides. Furtherly, 8 monosaccharides and oligosaccharides were analysed quantitatively using ion chromatography with good linear relationship (R2≥0.999 0). The results indicated that the contents of glucose, melibiose, galactose, mannose, manninotriose, stachyose and acteoside were different in the extracts of the fresh RR, crude RR and processed RR (p<0.05). The content of stachyose and fructose was the highest in fresh RR and raw RR, respectively. The other 6 saccharides trend to increase in processing, presumingthat the stachyose in fresh RR might havea multi-channel hydrolytic process. This study explored the regulation of the whole chemical components of fresh RR in the processing process and provided data support for laboratory investigation and the clinical application of fresh RR. Moreover, these data provided a reference for quality control of fresh Chinese herbal medicine and gave new ideas for traditional Chinese medicine processing research.
Key words:Fresh Radix Rehmanniae; Processing; Infrared spectroscopy; Ion chromatography
[1] Chinese Pharmacopoeia Commission (国家药典委员会). Pharmacopoeia of the People’s Republic of China(中华人民共和国药典), Part 1 (一部). Beijing: China Medical Science Press(北京:中国医药科技出版社), 2020. 129.
[2] CHEN Jin-peng, ZHANG Ke-xia, LIU Yi, et al(陈金鹏, 张克霞, 刘 毅, 等). Chinese Traditional and Herbal Drugs(中草药), 2021, 52(6): 1772.
[3] WANG Bo, LÜ Chen-zi, HE Mei-jing, et al(王 勃, 吕辰子, 何美菁, 等). World Science and Technology/Modernization of Traditional Chinese Medicine and Materia Medica(世界科学技术-中医药现代化), 2018, 20(6): 1010.
[4] CHEN Jin, TAN Li-yuan, LI Min, et al(陈 瑾, 谭丽媛, 李 敏, 等). Lishizhen Medicine and Materia Medica Research(时珍国医国药), 2018, 29(7): 1625.
[5] Xue Shujuan, Wang Lili, Chen Suiqing. Molecules,2018, 23(3): 541.
[6] TAN Li-yuan, CHEN Jin, WANG Xu-wen, et al(谭丽媛, 陈 瑾, 王旭文, 等). Chinese Traditional Patent Medicine(中成药), 2020, 42(9): 2520.
[7] ZHANG Bo-yong, JIANG Zhen-zuo, WANG Yue-fei, et al(张波泳, 江振作, 王跃飞, 等). Chinese Traditional Patent Medicine(中成药), 2016, 38(5): 1104.
[8] FAN Ke-feng, TANG Fa-yin, ZHAO Jian-ping, et al(樊克锋, 汤法银, 赵建平, 等). Chinese Journal of Veterinary Drug(中国兽药杂志),2017, 51(3): 55.
[9] Zhao Ping, Li Xia, Wang Ying, et al. Carbohydrate Polymers,2020, 233: 115836.
[10] LI Wen-xia, WANG Xiu-zhen, PEI Yuan-yuan, et al(李雯霞, 王秀真, 裴圆圆, 等). Asia-Pacific Traditional Medicine(亚太传统医药), 2020, 16(12): 36.
[11] WU Tang(吴 瑭). Detailed Analysis of Epdemic Warm Diseases(温病条辨). Beijing: People’s Medical Publishing House(北京: 人民卫生出版社),2005. 120.
[12] YAN Xi-ting(严西亭). De Pei Ben Cao(得配本草). Shanghai: Shanghai Scientific & Technical Publishers (上海: 上海卫生出版社), 1957. 17.
[13] LI Shi-zhen(李时珍). Compendium of Material Medica(本草纲目). Beijing: People’s Medical Publishing House(北京: 人民卫生出版社),1977. 89.
[14] LIANG Ai-hua, XUE Bao-yun, WANG Jin-hua, et al(梁爱华, 薛宝云, 王金华, 等). China Journal of Chinese Material Media(中国中药杂志), 1999, 24(11): 663.