Interaction between Low Molecular Weight Chitosan Derivatives and Bovine Serum Album
HUANG Yan1, LIU Yun1, ZHANG Qi1, 2*, JIA Chun-man2, HUA Ming-qing1, CAO Juan1, ZHU Wei-hua1
1. School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China
2. Hainan Provincial Key Lab of Fine Chemicals, Haikou 570228, China
Abstract：The effect between biocompatible low molecular weight chitosan derivatives as the delivery carrier of active pharmaceuticals bovine serum album (BSA) was studied. Three derivatives of low molecular weight chitosan with 20 degree of polymerization (NLCS20) and narrow molecular weight distribution including N-maleoyl-NLCS20, N-carboxymethyl-NLCS20 and N-succinyl-NLCS20 were synthesized. The effect between NLCS20 derivatives and BSA was studied with UV spectrum, fluorescence spectrum and isothermal titration calorimetry (ITC). The results showed that the conformation and activity of BSA did not change a lot in the microenvironment of NLCS20 derivatives solution. The process was exothermic and spontaneous. The main binding force was hydrogen bond and hydrophobic interaction. It was concluded that low molecular weight derivatives are likely to be delivery carriers of hydrophilic drugs such as active protein or poly amino acid.
[1] Borchard G, Luessen H L, De Boer A G, et al. Journal of Controlled Release, 1996, 39(2-3): 131.
[2] Yolles S, Eldridge J E, Woodland J H R. Polymers News, 1971, 1: 9.
[3] Kajihara M, Sugie T, Hojo T, et al. Journal of Controlled Release, 2001, 73(2-3): 279.
[4] Liu W G, Zhang X, Sun S J, et al. Bioconjugate Chemistry, 2003, 14(4): 782.
[5] Artursson P, Lindmark T, Davis S S, et al. Pharmaceutical Research, 1994, 11(9): 1358.
[6] Hu Y, Jiang X Q, Ding Y, et al. Biomaterials, 2002, 23(15): 3193.
[7] Dureja H, Tiwary A K, Gupta S, et al. International Journal of Pharmaceutics, 2001, 213(1-2): 193.
[8] Zhu A P, Yuan L H, Chen T, et al. Carbohydrate Polymers, 2007, 69(2): 363.
[9] Kasaai M R. Carbohydrate Polymers, 2008, 71(4): 497.
[10] Huang Y, Zhang X Y, Zhang Q, et al. Magnetic Resonance Imaging, 2011, 29(4): 554.
[11] SUN Tao, YIN Xu-hong, GAN Jian-hong, et al(孙 涛, 银旭红, 甘建红, 等). Natural Product Research and Development(天然产物研究与开发), 2010, 22(5): 890.
[12] Kasai S, Horie T, Mizuma T, et al. Journal of Pharmaceutical Sciences, 1987, 76(5): 387.
[13] Gao H, Wang Y N, Fan Y G, et al. Bioorganic & Medicinal Chemistry, 2006, 14(1): 131.
[14] Kandori K, Uoya Y, Ishikawa T. Journal of Colloid and Interface Science, 2002, 252(2): 269.
[15] Lindman S, Lynch I, Thulin E, et al. Nano Letters, 2007, 7(4): 914.
