Analysis and Identification of H. rhamnoides subsp. sinensis Harvested From Different Producing Areas by FTIR Spectroscopy
LIU Yue1, LI Jing-yi2, FAN Gang1, LIU Chuan1, SUN Su-qin3, SU Yong-wen1, JIANG Dao-feng1, ZHANG Yi1*, TU Ya4*
1. College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China 2. Department of Traditional Chinese Pharmacy, Harbin University of Commerce, Harbin 150001, China 3. Department of Chemistry, Tsinghua University, Beijing 100084, China 4. China Academy of Chinese Medical Sciences, Beijing 100700, China
Abstract：The accurate identification of traditional Chinese medicine (TCM) which collected from different producing areas is important for its quality control and clinical effects. In the present study, Fourier transform infrared spectroscopy (FTIR) combined with second derivative spectra were used to identify and analyze H. rhamnoides subsp. sinensis from different producing areas. The characteristic absorption peaks, including 2 925, 2 854, 1 743, 1 541 and 1 173 cm-1 belonging to fatty acids, flavonoids and saccharides appear in all 20 samples. But the absorption peak intensities and locations varied due to the different geographical regions. The results also showed that the absorption peaks at the range of 3 429～3 336 and 1 744 cm-1 were important characteristic absorption peaks which can identify H. rhamnoides subsp. sinensis from different producing areas. Also, absorption peaks at 1 030 and 1 516 cm-1 further confirmed the existence of flavonoids in all samples by comparing the second derivative infrared spectra in the range of 1 800～1 000 cm-1. However, the samples’ differences can be intuitively found around peaks 1 711, 1 476 cm-1 and ranges from 1 689～1 515 and 1 400～1 175 cm-1. The results demonstrated that FTIR was a simple, convenient, fast and intuitive approach to identify and analyze H. rhamnoides subsp. sinensis from different producing areas. This method provides foundations for the analysis of chemical compositions and quality control for the TCM.
刘 悦1，李静怡2，范 刚1，刘 川1，孙素琴3，苏永文1，江道峰1，张 艺1*，图 雅4* . 不同产地中国沙棘的傅里叶变换红外光谱识别研究 [J]. 光谱学与光谱分析, 2016, 36(04): 948-954.
LIU Yue1, LI Jing-yi2, FAN Gang1, LIU Chuan1, SUN Su-qin3, SU Yong-wen1, JIANG Dao-feng1, ZHANG Yi1*, TU Ya4*. Analysis and Identification of H. rhamnoides subsp. sinensis Harvested From Different Producing Areas by FTIR Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(04): 948-954.
[1] Yutuoningma·Yuandangongbu(允妥宁玛·云丹贡布). Four-Volume Medical Code(四部医典). Beijing: People’s Medical Publishing House(北京：人民卫生出版社), 1983. [2] MA Shi-lin, MAO Ji-zu(马世林, 毛继祖，译注). Yue Wang Yao Zhen(月王药诊). Shanghai: Shanghai Science and Technology Press(上海: 上海科技出版社), 2012. [3] Dimaer·Danzengpengcuo(帝玛尔·丹增彭措). Jing Zhu Materia Medica(晶珠本草). Beijing: Nationalities Publishing House(北京: 民族出版社), 2005. 65. [4] Zhanbula·Daoerji (占布拉·道尔吉). Classic Canon of Mongolian Materia Medica(蒙药正典). Beijing: Nationalities Publishing House(北京: 民族出版社), 2006. [5] Chinese Pharmacopoeia Commission(国家药典委员会): The Pharmacopoeia of the People’s Republic of China(中华人民共和国药典). Beijing: China Medical Science Press(北京: 中国医药科技书版社), 2010. 171. [6] CHEN Chu, ZHANG Hao, XIAO Wei(陈 雏, 张 浩, 肖 蔚). The Global Seabuckthorn Research and Development(国际沙棘研究与开发), 2005, 3(4): 25. [7] Adiana Mohamed Adib, Fadzureena Jamaludin, Ling Sui Kion, et al. Journal of Pharmaceutical and Biomedical Analysis, 2014, 96: 104. [8] Dugeer, MA Chun-jie, CAI Qiu-jie, et al(都格尔, 麻春杰, 蔡秋杰, 等). World Science and Technology-Modernization of Traditional Chinese Medicine and Materia Medica(世界科学技术-中医药现代化), 2014, 16(3): 661. [9] Li Jianrui, Sun Suqin, Wang Xiaoxiao, et al. Journal of Molecular Structure, 2014, 1069: 229. [10] ZHANG Hai-hong, ZHANG Shu-juan, WANG Feng-hua, et al(张海红, 张淑娟, 王凤花, 等). Acta Optica Sinica(光学学报), 2010, 30(2): 574. [11] Wu Xianxue, Xu Changhua, Li Ming, et al. Journal of Molecular Structure, 2014, 1069: 133. [12] LEI Yu, TU Ya, ZHANG Yan-ling, et al(雷 雨, 图 雅, 张艳玲, 等). Analytical Instrumentation(分析仪器), 2010, 3: 24. [13] Li Mingyu, Cheng Shichao, Li Dan, et al. Chinese Chemical Letters, 2015, 26: 221. [14] Alam Zeb. Important Therapeutic Uses of Sea Buckthorn (<i>Hippophae</i>): A Review. Journal of Biological Sciences, 2004, 4(5): 687.
