Rapid Determination of the Multi-Marker Ingredients in Heterosmilacis Japonicae Rhizoma and Sophorae Flavescentis Radix with Near-Infrared Diffused Reflection Spectroscopy
ZHAO Feng-chun, WAN Kai-yang, LIU Xiao-qian, ZHANG Qi-wei, GAO Hui-min*, WANG Zhi-min*
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine, Beijing 100700, China
Abstract:A rapid NIRS method for determination of macrozamin in Heterosmilacis japonicae rhizoma (HJR), and the total content of oxymatrine and matrine (OMT+MT) as well as the total content of oxysophocarpine and sophocarpine (OSC+SC) in sophorae flavescens radix (SFR) was developed to explore the application feasibility of NIRS for the quality assurance system of Chinese patent drugs. The contents of macrozamin in HJR samples, and OMT+MT and OSC+SC in SFR samples were determined by HPLC as reference values. The NIR spectra of the samples were measured in a diffused reflection mode. The different characteristic wavebands and pretreatment methods were optimized. The quantitative calibration models between the NIR spectra and the content reference values of marker components in HJR and SFR samples, were established with partial least square method, and further optimized through the cross validation and external validation. The contents of macrozamin in 88 batches of HJR samples were over the range of 0.36~12.88 mg·g-1. The total contents of OMT+MT and OSC+SC in 75 batches of SFR samples were over the range of 8.87~66.31 and 2.30~15.11 mg·g-1, respectively. The performance of the final models for macrozamin, OMT+MT and OSC+SC was evaluated well according to correlation coefficients (r), root mean square error of cross-validation (RMSECV) and root mean square error of prediction (RMSEP). The R2 values of the cross-validation for macrozamin, OMT+MT and OSC+SC were 0.902 5, 0.949 1 and 0.913 7, and those of RMSECV were 0.961, 2.45 and 0.724 mg·g-1 respectively. The R2 values of external validation for the three models were 0.981 7, 0.982 6 and 0.960 9, and those of RMSEP were 0.693, 2.27 and 0.658 mg·g-1, respectively. This is the first report on rapid determination of macrozamin in Heterosmilacis japonicae rhizoma, and oxymatrine, matrine, oxysophocarpine and sophocarpine in sophorae flavescens radix by NIRS method. The presented method can fulfill the requirement of rapid acquirement of chemical information of raw medicinal materials prior the manufacturing of compound Kushen injection.
赵凤春,万楷杨,刘晓谦,张启伟,高慧敏*,王智民*. 近红外漫反射光谱法快速测定苦参和白土苓药材中多指标成分的含量[J]. 光谱学与光谱分析, 2014, 34(10): 2652-2656.
ZHAO Feng-chun, WAN Kai-yang, LIU Xiao-qian, ZHANG Qi-wei, GAO Hui-min*, WANG Zhi-min*. Rapid Determination of the Multi-Marker Ingredients in Heterosmilacis Japonicae Rhizoma and Sophorae Flavescentis Radix with Near-Infrared Diffused Reflection Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(10): 2652-2656.
[1] TU Yao-sheng, LIU Jun, ZHANG Jian-jun(涂瑶生,柳 俊,张建军). China Journal of Chinese Materia Medica (中国中药杂志), 2011, 36(17): 2433. [2] WAN Kai-yang, GAO Hui-min, ZHANG Qi-wei, et al(万楷杨,高慧敏,张启伟,等). Chinese Journal of Experimental Traditional Medical Formulae(中国实验方剂学杂志), 2011, 17(10): 56. [3] MA Yue, ZHANG Qi-wei, GAO Hui-min, et al(马 悦,张启伟,高慧敏,等). Chinese Journal of Experimental Traditional Medical Formulae(中国实验方剂学杂志), 2012, 18(23): 342. [4] Ma Yue, Gao Huimin, Liu Jing, et al. Journal of Liquid Chromatography & Related Technologies, 2014, 37: 207. [5] China Pharmacopoeia Commission(国家药典委员会). Pharmacopoeia of the People’s Republic of China (Vol.1) (中华人民共和国药典,1 部). Beijing: Chemical Industry Press( 北京: 化学工业出版社), 2010. 188. [6] GONG Xiao-yu, YIN Li-hui, YU Chi, et al(巩晓宇,尹利辉,余 驰,等). Chinese Journal of Pharmaceutical Analysis(药物分析杂志),2010, 30(10): 1935. [7] CHEN Chen, LI Wen-long, QU Hai-bin, et al(陈 晨,李文龙,瞿海斌,等). Chinese Journal of Pharmaceutical Analysis(药物分析杂志),2010, 32(10): 1781.