Comparison of Discriminative Methods of Hypoglycemic Drugs by Raman Spectroscopy
GAO Qun1, ZHANG Zhong-hu2, LU Feng1*
1. College of Pharmacy, Second Military Medical University, Shanghai 200433, China 2. Shandong Provincial Institute for Food and Drug Control, Ji’nan 250012, China
Abstract:In the present paper, four kinds of cluster analysis methods were used in rapid, non-destructive discrimination of hypoglycemic tablets by the Raman spectroscopy technology. Nine kinds of hypoglycemic tablets, including 48 samples, were determined using a Raman spectrometer. The sample data were pretreated with the methods of frequency range cutting, baseline correction, smoothing and vector normalization, then were analyzed by K-means, hierachical cluster, self-organizing maps(SOM) and PCA-SOM respectively. The results demonstrated that SOM was better than K-means and hierachical cluster, and it provided the best discrimination when combined with PCA. The research offers a new approach to the rapid discrimination of different kinds of hypoglycemic tablets.
[1] FAN Xian-song, ZHANG Xian-fen, SHEN Gao-shan(范贤松, 张先芬, 沈高山). Anhui Medical and Pharmaceutical Journal(安徽医药), 2010, 14(12): 1501. [2] Veij M, Deneckere A, Vandenabeele P, et al. Pharmaceutical Biomedical Analalysis, 2008, 46: 303. [3] WANG Yue-qing, YANG Wei(王跃卿, 杨 威). China Medical Herald(药品鉴定), 2010, 7(13): 56. [4] Rajalahti T, Kvalheim O M. International Journal of Pharmaceutics, 2011, 417: 280. [5] Rodionova O Y, Pomerantsev A L. Trends in Analytical Chemistry, 2010, 29(8): 795. [6] Been F, Roggo Y, Degardin K, et al. Forensic Science International, 2011, 211: 83. [7] Roggo Y, Degardin K, Margot P. Talanta, 2010, 81: 988. [8] de Peinder P, Vredenbregt M J, Visser T, et al. Journal of Pharmaceutical and Biomedical Analysis, 2008, 47: 688. [9] MENG Jia-na, DENG Li-ling, YU Yu-hai, et al(孟佳娜,邓俐伶,于玉海,等). Journal of Dalian Nationalities University(大连民族学院学报), 2011,13(3): 274. [10] SHI You-ming, LIU Gang, SUN Yan-lin, et al(时有明,刘 刚,孙艳琳,等). The Journal of Light Scattering(光散射学报), 2010, 22(2): 171. [11] ZHOU Shao-hua, FU Lue, LIANG Bao-liu(周少华 ,付 略,梁宝鎏). Science in China Press(中国科学), 2008, 38(7): 1089. [12] ZHAO Peng-yi, GENG Huan-tong, CAI Qing-sheng, et al(赵鹏一,耿焕同,蔡庆生, 等). Computer Technology and Development(计算机技术与发展), 2007, 7(9): 35. [13] LIU Tian-fei, TANG Guo-qing, LI Xue-wei(刘天飞,唐国庆,李学伟). Acta Veterimaria et Zootechnica Sinica(畜牧兽医学报), 2009, 40(2): 180. [14] JIA Rui-yu, GENG Jin-wei, NING Zai-zao, et al( 贾瑞玉,耿锦威,宁再早,等). Computer Engineeringand Applications(计算机工程与应用), 2010, 46(33): 121. [15] ZHANG Ying, PAN Bao-zhi(张 莹, 潘保芝). Well Logging Technology(测井技术), 2009, 33(6): 550.