Raman Spectroscopic Study on the Influence of Ultraviolet-A Radiation on CollagenⅠ
MA Rong-tian, GUO Zhou-yi*, LIU Zhi-ming, ZHONG Hui-qing, ZHUANG Zheng-fei, ZHAI Juan
MOE Key Laboratory of Laser Life Science & Laboratory of Photonic Chinese Medicine, College of Biophotonics, South China Normal University, Guangzhou 510631, China
Abstract：Raman spectroscopy was used to study the influence of ultraviolet-A(UV-A) radiation on collagenⅠ. The Raman spectra of collagenⅠ and that after 90 min UV-A radiation were reported. The results proved that irradiation with 90 min UV-A caused the change in the structures of collagenⅠ. Intramolecular hydrogen bonds were broken, and the hydrogen bonding system was changed. The intensity of helix was decreased, while the intensity of the disordered conformation in proteins such as random coil was increased. Otherwise，the UV-A radiation influenced the hydroxylation of proline and the content of hydroxyproline was reduced. The changes caused by UV-A radiation could damage the triple helical structure of collagenⅠ. It would lead to a series of changes，such as the destruction of collagen fibers during the photoaging of skin.
马荣甜，郭周义*，刘智明，钟会清，庄正飞，翟 娟 . 长波紫外辐射对Ⅰ型胶原损伤的拉曼光谱研究[J]. 光谱学与光谱分析, 2012, 32(02): 383-385.
MA Rong-tian, GUO Zhou-yi*, LIU Zhi-ming, ZHONG Hui-qing, ZHUANG Zheng-fei, ZHAI Juan . Raman Spectroscopic Study on the Influence of Ultraviolet-A Radiation on CollagenⅠ . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(02): 383-385.
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