Abstract:The L-theanine was tested using confocal Raman microscopy. Obvious Raman bands were showed in the range of 250~1 700 and 2 800~3 000 cm-1. The Raman bands were assigned with a preliminary analysis and the characteristic vibrational modes were gained in different range of wave numbers. Eight strong Raman bands were observed in the Raman spectra at 321, 900, 938, 1 153, 1 312, 1 358, 1 454 and 1 647 cm-1, respectively. They are the characteristic Raman bands of L-theanine. The results showed that Raman spectroscopy might be a new kind of precise, direct and fast detecting method for theanine.
[1] SHUAI Yu-ying, ZHANG Tao, JIANG Bo, et al(帅玉英,张 涛,江 波,等). Food and Fermentation Industries(食品和发酵工业), 2008, 34(11): 117. [2] Mary Bedner, Lane C Sander, Katherine E Sharpless. Analytical and Bioanalytical Chemistry, 2010, 397: 1773. [3] Liu Qian, Duan Huiying, Luan Jinling, et al. Cytotechnology, 2009, 59(3): 211. [4] Di X, Yan J, Zhao Y, et al. Neuroscience, 2010, 168(3): 778. [5] YANG Xu-gang, WU Qi-lin(杨序纲,吴琪琳). Raman Spectroscopy Analysis and Application(拉曼光谱的分析与应用). Beijing: National Defense Industry Press(北京: 国防工业出版社), 2008. 4. [6] Lin Juqiang, Chen Rong, Feng Shangyuan, et al. Biosensors and Bioelectronics, 2009, 25(2): 388. [7] Feng Shangyuan, Chen Rong, Lin Juqiang, et al. Biosensors and Bioelectronics, 2010, 25(11):2414. [8] Santosh Kumar, Amareshwar Kumar Rai, Rai S B, et al. Journal of Molecular Structure, 2006, 791(1~3): 23. [9] Moreno A J D, Freire P T C, Guedes I, et al. Brazilian Journal of Physics, 1999, 29(2): 380. [10] Casado J, Lopez Navarrete J T, Ramirez F J. Journal of Raman Spectroscopy, 1995, 26: 1003. [11] Ramírez F J, Tuón I, Silla E. Journal of Physical Chemistry B, 1998, 102(32): 6290. [12] Dhamelincourt P, Ramirez F J. Applied Spectroscopy, 1993, 47(4): 446. [13] López Navarrete J T, Hernández V, Ramírez F J. Journal of Molecular Structure, 1997, 410~411: 353. [14] López Navarrete J T, Hernández V, Ramírez F J. Journal of Molecular Structure, 1995, 348: 249. [15] ZHOU Wen, CHEN Xin, SHAO Zheng-zhong(周 文,陈 新,邵正中). Progress in Chemistry(化学进展), 2006, 18(11): 1514. [16] Dollish F R, Fateley W G, Bentley F F. Characteristic Raman Frequencies of Organic Compounds(有机化合物的特征拉曼频率). Translated by ZHU Zi-ying(朱自莹,译). Beijing: Chinese Chemical Society(北京: 中国化学会), 1980.