Abstract:In the present paper, the interaction between ambroxol hydrochloride (ABX) and human serum albumin (HSA) was studied under simulative physiological condition by spectroscopy and molecular modeling method. Stern-Volmer curvers at different temperatures and UV-Vis absorption spectroscopy showed that ABX quenched the fluorescence of HSA mainly through dynamic quenching mode. On the basis of the thermodynamic data, the main binding force between them is hydrophobic interaction. According to the theory of Frster non-radiation energy transfer, the binding distance between the donor and the acceptor was 3.01 nm. The effect of ABX on the conformation of HSA was analyzed by the synchronous and three-dimensional fluorescence spectroscopy. Furthermore, using the molecular modeling method, the interaction between them was predicted from molecular angle: ABX might locate in the subdomain ⅢA of HSA.
梁 晶,冯素玲*. 光谱法和分子对接法研究盐酸氨溴索与人血清白蛋白的相互作用 [J]. 光谱学与光谱分析, 2011, 31(04): 1020-1024.
LIANG Jing, FENG Su-ling*. Interaction between Ambroxol Hydrochloride and Human Serum Albumin Studied by Spectroscopic and Molecular Modeling Methods . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(04): 1020-1024.
[1] XU Jin-gou, WANG Zun-ben(许金钩, 王尊本). Fluorescence Analysis Method(荧光分析法). Beijing: Science Press(北京: 科学出版社), 2006. 64. [2] YANG Pin, GAO Fei(杨 频, 高 飞). Theory of Bioinorganic Chemistry(生物无机化学原理). Beijing: Science Press(北京: 科学出版社), 2002. 311. [3] Ross P D, Subramanian S. Biochemistry, 1981, 20: 3096. [4] Frster T. Modern Quantum Chemistry. New York: Academic Press, 1996. 93. [5] Cyril L, Earl J K, Sperry W M. Biochemists Handbook. London: Epon Led. Press, 1961. 83. [6] Valeur B, Brochon J C. New Trends in Fluorescence Spectroscopy, 6th ed. Berlin: Springer Press, 1999. 25. [7] Brustein E A, Vedenkina N S, Irkova M N. Photochemistry Photobiology, 1973, 18(4): 263. [8] Honore B, Pedersen A O. Biochemistry, 1989, 28: 199. [9] Ndou T T, Warner I M. Chem. Rev., 1991, 91: 493. [10] Carter D C, Ho J X. Adv. Protein Chem., 1994, 45: 153. [11] Sudlow G, Birkett D J, Wade D N. Mol. Pharmacol., 1975, 11: 824. [12] Sudlow G, Birkett D J, Wade D N. Mol. Pharmacol., 1976, 12: 1052.