Relationship of Zn Content and Na/K Ratio to the Resistance to Pathogen Invasion in Antisense ACS Transgenic Tomato Fruit and Normal Tomato
FAN Bei1,SHEN Lin1,2,LIU Can1,TIAN Shi-ping2,SHENG Ji-ping1*
1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China 2. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China
Abstract：In the present work, inductively coupled plasma atomic emission spectrometry (ICP-AES) was used to determine the content of Zn, Na and K and the ratio of Na/K in antisense ACS transgenic tomato fruit (in which the antisense ACC synthase gene construct was inserted) and in normal tomato fruit of the same variety (Lycopersicon esculentum cv. Lichun). The difference in mineral elements content between the two tomatoes and the involvement of Zn content and Na/K ratio in the disease resistance of postharvest tomato fruit were discussed. The results showed that the ethylene biosynthesis is limited in antisense ACS transgenic tomato fruit. Meanwhile, the resistance to Rhizopus nigricans invasion of this transgenic tomato was higher, the disease incidence and lesion area in transgenic tomato fruit were 20.0% and 33.3% lower than those in control fruit, respectively. In addition, the content of Zn in transgenic tomato fruit was 0.322 μg·g-1 and was 1.5 times higher than that in Lichun tomato fruit. There was no significant difference in K content between transgenic tomato fruit and Lichun fruit, but the content of Na in transgenic tomato fruit was significant higher than that in Lichun fruit and the Na/K ratio in transgenic tomato fruit was 2.0 times higher than that in Lichun fruit. It is suggested that the content of Zn and the Na/K ratio may be involved in the resistance response to pathogen invasion and the development of antisense ACS transgenic tomato fruit.
范蓓1,申琳1,2,刘灿1,田世平2,生吉萍1*. 转反义ACS基因番茄和普通番茄果实中锌含量和钠钾比与抗病性的关系研究[J]. 光谱学与光谱分析, 2009, 29(02): 492-495.
FAN Bei1,SHEN Lin1,2,LIU Can1,TIAN Shi-ping2,SHENG Ji-ping1*. Relationship of Zn Content and Na/K Ratio to the Resistance to Pathogen Invasion in Antisense ACS Transgenic Tomato Fruit and Normal Tomato . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(02): 492-495.
[1] Masn H(马斯纳 H著). Mineral Substance Nourishment of Higher Plant: Soil and Plant Nutrition(高等植物的矿质营养). Translated by CAO Yi-ping(曹一平, 译). Beijing: Beijing Agricultural University Press(北京: 北京农业大学出版社), 1991. 223. [2] Arafa M A. Agricultural Research Review, 1985, 63(2): 29. [3] Rossbauer G. Cause and Possibilities for Control, 1986, 37(4): 47. [4] WANG Xiao-ming, WU An-quan, ZHANG Pei-kun(王晓明, 吴安全, 张培坤). Plant Protection(植物保护), 1999, 25(2): 23. [5] Sharma S R, Kolte S. J. Plant and Soil, 1994, 167: 313. [6] ZHAO Mingui, TIAN Qiuying, ZHANG Wenhao. Plant Physiology, 2007, 144: 206. [7] TAN Qi-ling, WANG Yun-hua, WU Li-shu(谭启玲, 王运华, 吴礼树). Journal of Huazhong Agricultural University(华中农业大学学报), 1995, 14(2): 154. [8] XIONG Zai-hui, DENG Bai-ping(熊再辉, 邓白平). Journal of Hunan Agricultural University(湖南农业大学学报), 1996, 22(2): 147. [9] LUO Yun-bo, SHENG Ji-ping, SHEN Lin(罗云波, 生吉萍, 申 琳). Journal of Agricultural Biotechnology(农业生物技术学报), 1995, 3(2): 38. [10] McKeon T A, Hoffman N E, Yang S F. Planta, 1982, 155: 437. [11] HOU Man-ling(侯曼玲). Food Analysis(食品分析). Beijing: Chemical Industry Press(北京: 化学工业出版社), 2004. 23, 151. [12] LI Shun-jiang, YANG Lin-sheng, WANG Wu-yi, et al(李顺江, 杨林生, 王五一, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(3): 585. [13] SUN Yong, YANG Gang, ZHANG Jin-ping(孙 勇, 杨 刚, 张金平, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(2): 371. [14] ZHANG Yan-cai, ZHOU Xiao-fen, LI Qiao-yun, et al(张彦才, 周晓芬, 李巧云, 等). Plant Nutrition and Fertilizer Science(植物营养与肥料学报), 2006, 12(1): 115. [15] MU Kang-guo, ZHAO Xiu-qin, LI Jiang-qiang, et al(慕康国, 赵秀琴, 李健强, 等). Journal of China Agricultural University(中国农业大学学报), 2000, 5(1): 84.
