摘要： The fluorescence characteristics of the complex of piperine with cation in micellar system were studied. At the same time, the influence of experimental condition on the fluorescence intensity was also studied. The experiments indicated that piperine had very low fluorescence signal itself. But in pH 8.77 Na2HPO4-NaH2PO4 buffer solution, both Mn(Ⅱ) and cationic surfactant cetyltrimethylammonium bromide can enhance the fluorescence intensity and stability of piperine. Based on this, a sensitive method has been developed for the quantitative determination of piperine in Mn(Ⅱ)-piperine-cetyltrimethylammonium bromide ternary system. Under the optimum conditions, there is a linear relationship between the enhancement of fluorescence intensity and the concentration of Mn(Ⅱ). The optimal conditions are as follows: the concentration of Mn(Ⅱ) is 2.5×10-4 mol·L-1 and the concentration of cetyltrimethylammonium bromide is 5×10-4 mol·L-1. The fluorescence intensity was determined by a 1 cm quartz cell with the excitation wavelength of 352 nm and the emission wavelength of 452 nm. The linear range of concentration of piperine was 2.02-10.1 μg·mL-1 with the relative coefficient of 0.998 5 and the detection limit of 0.060 2 μg·mL-1. The relative standard deviation (RSD) was 1.10%. The proposed method has been successfully applied to the quantitative determination of piperine in the Mongolian medicine Piper longum L, and the recovery was within the range of 97.6%-102.0%. The results were very satisfactory.
[1] WEI Kun，DOU De-qiang，PEI Yu-ping，et al(韦 琨，窦德强，裴玉萍，等). China Journal of Chinese Materia Medica(中国中药杂志)，2002，27(5)：328. [2] JIA Fu-qun，BAI Wei(贾福群，白 炜). Chinese Traditional and Herbal Drugs(中草药)，2003，34(6)：527. [3] LI Jian，Lü Gui-bao (李 建，吕归宝). Chinese Traditional and Herbal Drugs(中草药)，2003，34(2)：139. [4] Waldemar Ternes , Edburga L Krause. Anal. Bioanal. Chem., 2002，374：155. [5] Verzele M, Qureshi S. Chromatographia, 1980, 13(4)：241. [6] XU Liang，XI Hai-shan，BI Kai-shun，et al(许 良，席海山，毕开顺，等). Chinese Traditional Patent Medicine(中成药)，2006，28(4)：603. [7] WEN Ai-ping，SU Rui-lin(温爱平，苏瑞林). Chinese Journal of Pharmaceutical Analysis(药物分析杂志)，2005，25(12)：1466. [8] MENG Zhao-hui，WANG Zhen-hui，ZHOU Shu-ping(孟召辉，汪振辉，周漱萍). Chinese Journal of Analytical Chemistry(分析化学)，1994，22(12)：1263. [9] HAN Wen-bin，ZHANG Ke-kai，LIANG Yun-ai(韩文彬，张科凯，梁云爱). Chinese Pharmaceutical Affairs(中国药事)，1996，10(4)：267. [10] WANG Zhen-hui，ZHANG Qian-li，ZHOU Shu-ping (汪振辉，张钱丽，周漱萍). Analytical Laboratory(分析试验室)，1999，18(3)：31. [11] WANG Tao，DU Heng-qing，WANG Cong(王 滔，都恒青，王 淙). Chinese Journal of New Drugs and Clinical Remedies(中药新药与临床药理)，1998，9(3)：178. [12] LIU Shuan-di，SHI Yan，MENG Gen-da-lai(刘拴娣，石 岩，孟根达来). Chinese Traditional Patent Medicine(中成药)，2000，22(7)：475. [13] PAN Zu-ting，YU Jun-ping(潘祖亭，余军平). Chinese Journal of Analytical Chemistry(分析化学)，2004，32(3)：338. [14] TONG Shang-lun，XIANG Guang-hong，LIU Wei-ping(童裳伦，项光宏，刘维屏). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005，25(12)：2061. [15] AODENG Gao-wa，ZHANG Ying，FAN Hai-yan(敖登高娃，张 莹，樊海燕). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006，26(8)：1530. [16] LIU Chun，ZHAO Hui-chun，JIN Lin-pei(刘 春，赵慧春，金林培). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，1999，19(4)：569. [17] ZHANG Min，QIU Zhao-hui，CAO Yong，et al(张 敏，邱朝晖，曹 庸，等). Lishizhen Medicine and Materia Medica Research (时珍国医国药)，2005，16(3)：238. [18] CHEN Guo-zhen, HUANG Xian-zhi，ZHENG Zhu-zi, et al(陈国珍，黄贤智, 郑朱梓，等). Fluorescence Analysis Method(Third Edition)(荧光分析法, 第3版). Beijing：Science Press(北京：科学出版社)，2006. 55. [19] The Pharmocopoeia Committee of the People’ s Republic of China(国家药典委员会编). Chinese Pharmacopoeia(中国药典). Beijing：Chemical Industry Press(北京：化学工业出版社)，2000. 188.
