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| The Interaction between 4-Ethyl-2-Methoxyphenol and Human Serum Albumin Studied by Spectroscopic and Molecular Docking Techniques |
| GONG Han-qing, CHEN Jian-bo* |
| School of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China |
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Abstract 4-Ethyl-2-methoxyphenol is a widely used food additive, but it also has toxicity. In order to investigate the effect of 4-Ethyl-2-methoxyphenol on the body, the interaction between 4-Ethyl-2-methoxyphenol and human serum albumin (HSA) was studied by combining a variety of spectroscopic techniques and molecular simulation techniques in this paper. UV absorption spectra results indicated that 4-Ethyl-2-methoxyphenol formed a new complex with human serum albumin. In addition, the fluorescence spectra results showed that the presence of 4-Ethyl-2-methoxyphenol can enhance the fluorescence intensity of HSA. 15 nm synchronous fluorescence and fluorescence enhancement effect equations can be used to calculate the binding constants between 4-Ethyl-2-methoxyphenol and HSA, and their binding constants decreased with the increasing of temperature. Thermodynamic parameters showed that 4-Ethyl-2-methoxyphenol was mainly bonded with HSA by hydrogen bonding and hydrophobic interaction. Moreover, synchronous fluorescence, three-dimensional fluorescence and circular spectra revealed that 4-Ethyl-2-methoxyphenol modified the conformation of HSA. The molecular docking technique demonstrated that 4-Ethyl-2-methoxyphenol was bonded in the IB hydrophobic region of HSA.
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Received: 2017-07-04
Accepted: 2017-11-09
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Corresponding Authors:
CHEN Jian-bo
E-mail: jianboch@shnu.edu.cn
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[1] Gallardo-Chacon J J，Karbowiak T. Food Chem., 2015, 181(15): 222.
[2] Tabassum S, Al-Asbahy W M, Afzal M, et al. Mol. Biosyst., 2012, 8: 2424.
[3] Yang H, Huang Y, Wu D, et al. New J. Chem., 2016, 40: 2530.
[4] Crump A M, Sefton M A，Wilkinson K L. Food Chem., 2014, 162: 261.
[5] Wang J, Ma L, Zhang Y, et al. Journal of Molecular Structure, 2017, 1129(2): 160.
[6] Poureshghi F, Ghandforoushan P, Safarnejad A, et al. J. Photochem Photobiol B, 2017, 166(7): 187.
[7] Lu Z, Qi L, Li G X, et al. Journal of Solution Chemistry, 2014, 43: 2010.
[8] Shahabadi N, Khorshidi A，Moghadam N. Spectrochim Acta A Mol. Biomol. Spectrosc.，2013, 114: 627.
[9] Wu F Y, Zhang L N, Ji Z J，et al. Journal of Luminescence，2010, 130: 1280.
[10] Azab H A, Mogahed E M, Awad F K, et al. J. Fluoresc., 2012, 22: 971.
[11] Bi S, Zhao T, Zhou H, et al. The Journal of Chemical Thermodynamics, 2016, 97: 113.
[12] Santos R L S R, Sanches R N F, de Oliveira Silva D. Journal of Coordination Chemistry，2015, 68: 3209.
[13] Zhao P, Gao G, Zhang L, et al. J. Pharm. Biomed. Anal., 2017, 141(15): 262.
[14] Shahraki S, Shiri F，Saeidifar M. Inorganica Chimica Acta，2017, 463: 80.
[15] Ali M S，Al-Lohedan H A. Journal of Molecular Liquids 2017, 236: 232.
[16] Abdullah S M S, Fatma S, Rabbani G，et al. Journal of Molecular Structure，2017, 1127: 283. |
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