光谱及等温滴定量热法探究盐酸阿霉素与DNA作用机理

doi:10.3964/j.issn.1000-0593(2018)02-0540-06

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Abstract The interaction processing of anti-cancer drug doxorubicin hydrochloride (DOX) with DNA was systematically investigated using fluorescence spectroscopy, ultraviolet-visible spectroscopy, infrared spectroscopy，circular dichroism and isothermal titration calorimetry measurements. The binding constant Ka, the binding-site number n, the reaction enthalpy ΔH and the reaction entropy ΔS of the interaction were obtained. In the binding process, the helix of B-DNA can have a certain degree of structural variations. The data of fluorescence spectrum indicated that DNA was a good quencher for the fluorescence of DOX. IR data certified the cation DOX⁺ binding with the phosphate groups of DNA through strong electrostatic attraction, while the C—H bonds of DOX may be associated with the bases of DNA by hydrophobic interaction. The Changes of enthalpy and entropy of the binding process were determined by ITC, which indicated that the process was driven by the hydrophobic interactions between the hydrocarbon chain of DOX and the bases of DNA.

Key words： Fluorescence spectroscopy Isothermal titration calorimetry Quencher；Binding constant Binding-site number

Received: 2016-07-02 Accepted: 2016-12-28

ZTFLH:

S481.1

Corresponding Authors: PU Xiao-hua E-mail: pxh913@163.com

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http://www.gpxygpfx.com/EN/10.3964/j.issn.1000-0593(2018)02-0540-06 OR http://www.gpxygpfx.com/EN/Y2018/V38/I02/540

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