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Exploration of the Interaction Mechanism between Doxorubicin Hydrochloride and DNA by Spectroscopic Techniques and Isothermal Titration Calorimetry |
WANG Huan, GOU Xing-xing, PU Xiao-hua*, WANG Jiao, HU Xiao-bing, LI Zong-xiao |
College of Chemistry and Chemical Engineering, Baoji University of Arts and Sciences, Baoji 721013, China |
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Abstract The interaction processing of anti-cancer drug doxorubicin hydrochloride (DOX) with DNA was systematically investigated using fluorescence spectroscopy, ultraviolet-visible spectroscopy, infrared spectroscopy,circular dichroism and isothermal titration calorimetry measurements. The binding constant Ka, the binding-site number n, the reaction enthalpy ΔH and the reaction entropy ΔS of the interaction were obtained. In the binding process, the helix of B-DNA can have a certain degree of structural variations. The data of fluorescence spectrum indicated that DNA was a good quencher for the fluorescence of DOX. IR data certified the cation DOX+ binding with the phosphate groups of DNA through strong electrostatic attraction, while the C—H bonds of DOX may be associated with the bases of DNA by hydrophobic interaction. The Changes of enthalpy and entropy of the binding process were determined by ITC, which indicated that the process was driven by the hydrophobic interactions between the hydrocarbon chain of DOX and the bases of DNA.
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Received: 2016-07-02
Accepted: 2016-12-28
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Corresponding Authors:
PU Xiao-hua
E-mail: pxh913@163.com
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[1] Doyle M L. Curr. Opin. Biotech.,1997, 8(1): 31.
[2] Nguyen B, Hamelberg D, Bailly C, et al. Biophys. J,2004, 86(2): 1028.
[3] Pitha J, Smid J. BBA-Biomembranes,1976, 425(3): 287.
[4] Luck A N, Mason A B. Adv. Drug. Deliver. Rev., 2013, 65(8): 1012.
[5] Rauf S, Gooding J, Akhtar K, et al. J. Pharmaceut. Biomed.,2005, 37(2): 205.
[6] Whittaker J, McFadyen W D, Baguley B C. Anti-Cancer. Drug. Des.,2001, 16(2): 81.
[7] Yarnell A T, Oh S, Reinberg D, et al. J. Biol. Chem.,2001, 276(28): 25736.
[8] Zhang J, Jian Y, Jun G, et al. Cell Res.,2004, 14(4): 283.
[9] Nishimura T, Okobira T, Kelly A M, et al. Biochemistry,2007, 46(27): 8156.
[10] Ding Y, Zhang L, Xie J, et al. J. Phys. Chem. B,2010, 114(5): 2033.
[11] Pérez-Arnaiz C, Busto N, Leal J M, et al. J. Phys. Chem. B,2014, 118(5): 1288.
[12] Chen P, Qiu M, Deng C, et al. Biomacromolecules,2015, 16(4): 1322.
[13] Johnson R P, Uthaman S, John J V, et al. Applied Materials & Interfaces,2015, 7(39): 21770.
[14] Tavano L, Muzzalupo R, Mauro L, et al. Langmuir.,2013, 29(41): 12638.
[15] Zunino F, Gambetta R, Di Marco A, et al. Biochimica et Biophysica Acta (BBA)-Nucleic Acids and Protein Synthesis,1977, 476(1): 38.
[16] Pyle A, Rehmann J, Meshoyrer R, et al. J. Am. Chem. Soc.,1989, 111(8): 3051.
[17] Cohen G, Eisenberg H. Biopolymers.,1969, 8(1): 45.
[18] Jangir D K, Tyagi G, Mehrotra R, et al. J. Mol. Struct.,2010, 969(1): 126.
[19] LIU Xiang-rong, SUN Xiu-chao, YANG Zai-wen, et al(刘向荣,孙秀超,杨再问,等). Chinese J. Inorg. Chem.(无机化学学报),2016, 32(2): 250.
[20] Ware W R. J. Phys. Chem. B,1962, 66(3): 455.
[21] Bi S, Song D, Kan Y, et al. Spectrochim. Acta A,2005, 62(1): 203.
[22] WANG Hui, GAN Guo-qing, QU Yang, et al(王 慧,甘国庆,瞿 阳,等). Chinese. J. Inorg. Chem.(无机化学学报),2012, 28(6): 1217.
[23] Raja D S, Bhuvanesh N S P, Natarajan K. Inorganica. Chimica Acta,2012, 385: 81.
[24] Fotticchia I, Fotticchia T, Mattia C A, et al. Langmuir.,2014, 30(48): 14427.
[25] Grüner S, Neeb M, Barandun L J, et al. BBA-Gen Subjects,2014, 1840(9): 2843.
[26] Jelesarov I, Bosshard H R. J. Mol. Recognit.,1999, 12(1): 3. |
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