| 光谱学与光谱分析 |
|
|
|
|
|
| Study on the Anisotropy of Collagenours Fibers in Articular with Infrared Imaging |
| WU Yue-chao, ZHANG Xue-xi, MAO Zhi-hua, YIN Jian-hua* |
| Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China |
|
|
|
|
Abstract Formalin solution has been widely used to solidify the organization of articular cartilage and prevent tissue decomposition in long-time measurement. However, it was rarely investigated that the structural anisotropy changes of collagen fiber (fixation) of articular cartilage when it was immersed in formalin. In this paper, Fourier transform infrared spectroscopic imaging with polarization technique was used to investigate the anisotropic structure change of collagen fiber of articular cartilage fixed in formalin through the absorbance change of Amide I and Amide II with immersing time and polarization direction. The degree of anisotropy of collagen fiber in cartilage was characterized with fitting related coefficient of absorbance. The anisotropy of Amide I and Amide II became stronger with immersing extension of articular cartilage in formalin, and the amide I showed more remarkable anisotropy. It was concluded that the formalin solution induced new crosslinks of collagen, which gradually strengthened the collagen fiber anisotropy and was helpful for the structural analysis of the articular cartilage. The study will be significant for the preparation, preservation and anisotropy research of cartilage specimen.
|
|
Received: 2015-06-01
Accepted: 2015-09-14
|
|
|
|
Corresponding Authors:
YIN Jian-hua
E-mail: yin@nuaa.edu.cn
|
|
[1] Maroudas A. Biorheology, 1975, 12(3-4): 233.
[2] Venn M, Maroudas A. Annals of the Rheumatic Diseases, 1977, 36(2): 121.
[3] Maroudas A. Nature, 1976, 260(5554): 808.
[4] Buckwalter J A, Mankin H J. Journal of Bone and Joint Surgery-American Volume, 1997, 79A(4): 600.
[5] Ramakrishnan N, Xia Y, Bidthanapally A, et al. Applied Spectroscopy, 2007, 61(12): 1404.
[6] Xia Y,! Zheng S K, Bidthanapally A. Journal of Magnetic Resonance Imaging, 2008, 28(1): 151.
[7] Camacho N P, Torzilli P A, Mendelsohn R, et al. Biopolymers, 2001, 62(1): 1.
[8] Yin J H, Xia Y, Xiao Z Y. Journal of Innovative Optical Health Sciences, 2013, 6(4): 1350048.
[9] Rieppo L, Narhi T, Helminen H J, et al. Journal of Biomedical Optics, 2013, 18(9): 1.
[10] Rieppo L, Saarakkala S, Narhi T, et al. Osteoarthritis and Cartilage, 2012, 20: 451.
[11] Fox C H, Johnson F B, Whiting J, et al. Journal of Histochemistry and Cytochemistry, 1985, 33(8): 845.
[12] Abe M, Takahashi M, Horiuchi K, et al. Analytical Biochemistry, 2003, 318(1): 118.
[13] Fishbein K W, Gluzband Y A, Kaku M, et al. Magnetic Resonance in Medicine, 2007, 57(6): 1000.
[14] Tan C I C, Sonia D, Kent G N, et al. Journal of Musculoskeletal Research, 2002, 6(2): 89.
[15] YIN Jian-hua, HUANG Feng-ling, QIAN Zhi-yu, et al(尹建华，黄凤玲，钱志余，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2014, 33(2): 340.
[16] Xia Y, Ramakrishnan N, Bidthanapally A. Osteoarthritis and Cartilage, 2007, 15(7): 780.
[17] Gadaleta S J, Landis W J, Boskey A L, et al. Connective Tissue Research, 1996, 34(3): 203.
[18] Coats A M, Hukins D W, Lmrie C T, et al. Journal of Microscopy, 2003, 211(1): 63.
[19] Bi X, Li G, Doty S B, et al. Osteoarthritis and Cartilage, 2005, 13(12): 1050.
[20] Ramakrishnan N, Xia Y, Bidthanapally A. Physics in Medicine and Biology, 2007, 52(15): 4601.
[21] Yin J H, Xia Y, Ramakrishnan N. Vibrational Spectroscopy, 2011, 57: 338.
[22] XIAO Zhi-yan, YIN Jian-hua(肖芝燕, 尹建华). The Journal of Light Scattering(光散射学报), 2014, 2: 213.
[23] Xia Y, Mittelstaedt D, Ramakrishnan N, et al. NIH Public Access, 2012, 74(2): 122. |
| [1] |
ZHONG Jing-jing1, 2, LIU Xiao1, 3, WANG Xue-ji1, 3, LIU Jia-cheng1, 3, LIU Hong1, 3, QI Chen1, 3, LIU Yu-yang1, 2, 3, YU Tao1, 3*. A Multidimensional Information Fusion Algorithm for Polarization
Spectrum Reconstruction Based on Nonsubsampled Contourlet
Transform[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(04): 1254-1261. |
| [2] |
SHI Dong-dong, CAO Zhao-bin, HUAN Yan-hua, GONG Yan-chun, WU Wen-yuan, YANG Jun*. Reflection Polarization Spectral Characteristics of High Performance Coating Material La2Zr2O7[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(10): 2995-2999. |
| [3] |
YANG Yun-han1, SHI Wei-xin2, QIU Jun-ting1. Study on Polarization Spectroscopy of Alteration Minerals[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 948-953. |
| [4] |
YU Miao1, LU Peng1*，CAO Xiao-wei1, TANG Ming-guang1, WANG Qing-kai1, LI Zhi-jun1, 2. Field Observations of the Bidirectional Reflectance Characteristics of Lake Ice[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(08): 2453-2461. |
| [5] |
MA Dan-ying, ZHAO Yuan, SHANG Lin-wei, ZHU Yong-kang, FU Juan-juan, LU Yan-fei, YIN Jian-hua*. Research Progress of Raman Spectroscopy Application for Articular Cartilage and Osteoarthritis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(07): 2029-2034. |
| [6] |
CHENG De-wei, LU Jing-qi*, JIA Xin-ting, WU Zi-jun, HUANG Jian. The Improvement of Signal-to-Back Ratio in Polarization Resolved Laser-Induced Breakdown Spectroscopy of Al-Fe Alloy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(01): 277-283. |
| [7] |
FENG Long, JIN Ke-xia, LIU Xing-e, TIAN Gen-lin, YANG Shu-min, JIANG Ze-hui, MA Jian-feng*. Study on Microfibrils Orientation in Daemonorops jenkinsiana Cell Wall by Polarized Laser Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(09): 2758-2762. |
| [8] |
LI Qing-ling1, 2, 3, YIN Da-yi1, 2, 3*. A Study of Spectral Polarization Properties of Oil Slick with Ellipsometry from Ultraviolet to Near-Infrared[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(06): 1661-1666. |
| [9] |
GAO Hao, ZHAI Ming-yang, SHANG Lin-wei, ZHAO Yuan, XU Hao, WANG Xiao, YIN Jian-hua*. Articular Cartilage Optical Clearing Research by Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(08): 2425-2429. |
| [10] |
WU Jin-hui1, 2, YU Jun-zhi1*, LIU Han-qi3, WANG Gao2, 4. Research on Polarization Spectrum Imaging System Based on Orthogonal Modulation[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2315-2319. |
| [11] |
WU Xi, PEI Xiao-jing, LIN Ruo-yun, LIU Feng, LI Na* . Fluorescence Anisotropy and Applications Based on Functional Nucleic Acid Recognition [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(01): 13-20. |
| [12] |
SUN Yao, WANG Hong . Analysis of Glass Surface Modification with Ion Beam Based on Ellipsometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(10): 3388-3393. |
| [13] |
QU Ying1, 2, LIU Qiang3, 4，LIU Su-hong2, 4 . A Forward Kernel Function for Fitting in situ Measured Snow Bidirectional Reflectance Factor[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(09): 2749-2754. |
| [14] |
MA Yu-ting1,2, ZHANG Jin1, SHI Chang-cheng2*, LI Wei2, HAN Xiao-hui1, PENG Xiao-yu2, WEI Dong-shan2, DU Chun-lei2, CUI Hong-liang1，2*. Current Status and Recent Advances in Research and Application of THz Technology in Articular Cartilage Detection [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(07): 2031-2035. |
| [15] |
CHENG Yu-qiong1, LU Wei1, 2*, HONG De-lin3, DANG Xiao-jing3, LUO Hui1 . Study on the Prediction of Germination Rate of Rice Seeds with Continuous Polarization Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(07): 2200-2206. |
|
|
|
|
