| 光谱学与光谱分析 |
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| In Situ Infrared Spectroscopic Determination of Enzyme Activity |
| REN Zhong-yuan, WU Yu-qing* |
| State Key Laboratory for Supramolecular Structure and Materials, Jilin University, Changchun 130012, China |
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Abstract A real-time infrared (IR) spectroscopy measurement is an effective means to obtain enzymatic information either in vitro or in living cells, as it can provide direct, continue test of biomacromolecule reactions. The principles of measurements are performed basing on the fact that the absorption bands in spectra of reactants and products are usually separated to each other and changed independently with time. Therefore, it is possible to measure the enzymatic efficiency at any reaction time according to the changes of characteristic band, from either reactant or product. That is, IR spectroscopy can be used to obtain intracellular structural information during the cells metabolic processes, as which can provide detailed and reliable scientific evidences. In this paper, we summarized the new developments of IR spectra in the in vitro enzymatic assay for several representative enzymes, as well the screening of enzyme inhibitors, which was further extended to the identical aspect by using living cells as detection model. Such important enzymatic examination closes to the physiological conditions without labeling, supplying structural information of the related biomolecules. The developing trends of IR spectra are discussed and the perspectives of it in the research area are also provided in this review.
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Received: 2014-09-02
Accepted: 2014-12-15
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Corresponding Authors:
WU Yu-qing
E-mail: yqwu@jlu.edu.cn
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[1] Michaelis L, Menten M L. Biochem., 1913, Z(49): 33.
[2] Soremsem S P L. Biochem., 1909, Z(21): 131.
[3] Hansn E H. Talanta, 1993, 40: 1891.
[4] Haris P I, Severcan F. J. Mol. Catalysis B: Enzymatic, 1999, 7: 207.
[5] Griffiths P R, Haseth J A. Chemical Analysis, 1986. 83.
[6] Meng Y, Yao C, Xue S, et al. Bioresour. Technol., 2014, 151: 347.
[7] Seeholzer S H, Jaworowski A, Rose I A. Biochem., 1991, 30: 727.
[8] Rose I A. J. Biol. Chem., 1970, 245: 6052.
[9] Kumar S, Barth A. Sensors, 2010, 10: 2626.
[10] Rudbeck M E, Kumar S, Mroginski M A, et al. J. Phys. Chem. A, 2009, 113: 2935.
[11] Barth A, Mntele W. Biophys. J., 1998, 75: 538.
[12] Takeuchi H, Murata H, Harada I. J. Am. Chem. Soc., 1988, 110: 392.
[13] Schindler R, Le Thannh H, Lendl B, et al. Vib. Spectrosc., 1998, 16: 127.
[14] Schomburg D, Salzmann M. Enzyme Handbook. Berlin: Springer-Verlag, 1991. pp. 3. 2. 1. 26.
[15] Karmali K, Karmali A, Teixeira A, et al. Anal. Biochem., 2004, 331: 115.
[16] López-Sánchez M, Ayora-Caada M J, Molina-Díaz A, et al. Anal. Bioanal. Chem., 2009, 394: 2137.
[17] Stolz M, Lewitzki E, Mntele W, et al. Biopolymers, 2006, 82: 368.
[18] Grell E, Schick E, Lewitzki E. Thermochim. Acta, 2001, 380: 245.
[19] Do L D, Buchet R, Pikula S, et al. Anal. Biochem., 2012, 430(1): 32.
[20] Imamura S, Horiuti Y. J. Biochem., 1979, 85: 79.
[21] Miller L M, Dumas P. Curr. Opin. Struct. Biol., 2010, 20: 649.
[22] Boydston-White S, Gopen T, Houser S, et al. Biospectroscopy, 1999, 5: 219.
[23] Lasch P, Pacifico A, Diem M. Biospectroscopy, 2002, 67: 335.
[24] Pacifico A, Chiriboga L, Lasch P, et al. Vib. Spectrosc., 2003, 32: 107.
[25] Matthus C, Bird B, Miljkovic M, et al. Methods. Cell. Biol., 2008, 89: 275.
[26] Manning G, Whyte D B, Martinez R, et al. Science, 2002, 298(5600): 1912.
[27] Matsuda M, Aoki K, Kiyokawa E, et al. Phil. Trans. R. Soc. B: Biol. Sci., 2008, 363(1500): 2143.
[28] Stukenberg P T, Fuller B G, Lampson M A, et al. Nature, 2008, 453(7198): 1132.
[29] Randriamampita C, Mouchacca P, Malissen B, et al. PLoS One, 2008, 3(1): 1521.
[30] Zhang J, Hupfeld C J, Taylor S S, et al. Nature, 2005, 437(7058): 569.
[31] Chen L, Holman H Y, Hao Z, et al. Anal. Chem., 2012, 84(9): 4118.
[32] Chalhoub N, Baker S J. Annu. Rev. Pathol., 2009, 4: 127.
[33] Suggit M, Bibby M. Clin. Can. Res., 2005, 11: 971.
[34] Travo A, Desplat V, Barron E, et al. Anal. Bioanal. Chem., 2012, 404: 1733.
[35] Desplat V, Geneste A, Begorre M, et al. J. Enzyme. Inhib. Med. Chem., 2008, 23: 648.
[36] Wolthuis R, Travo A, Nicolet C, et al. Anal. Chem., 2008, 80: 8461.
[37] Robey R, Hay N. Semin. Cancer. Biol., 2009, 19: 25.
[38] Vileno B, Jeney S, Sienkiewicz A, et al. Biophys. Chem., 2010, 152: 164.
[39] Lamoral-Theys D, Pottier L, Dufrasne F, et al. Curr. Med. Chem., 2010, 17: 812.
[40] Ramos S. J. Nutr. Biochem., 2007, 18: 427.
[41] Jagtap S, Meganathan K, Wagh V, et al. Curr. Med. Chem., 2009, 16: 1451.
[42] Yang C S, Wang X, Lu G, et al. Nat. Rev. Cancer., 2009, 9: 429.
[43] Derenne A, Van Hemelryck V, Lamoral-Theys D, et al. Biochim. Biophys. Acta, 2013, 1832(1): 46.
[44] Gasper R, Dewelle J, Kiss R, et al. Biochim. Biophys. Acta, 2009, 1788(6): 1263.
[45] Gasper R, Mijatovic T, Bénard A, et al. Biochim. Biophys. Acta, 2010, 1802(11): 1087.
[46] Gasper R, Vandenbussche G, Goormaghtigh E. Biochim. Biophys. Acta, 2011, 1808(3): 597.
[47] GAO Ti-yu, CI Yun-xiang（高体玉，慈云祥）. Process in Chemistry（化学进展）, 2008, 12(3): 346.
[48] LING Sheng-jie, SHAO Zheng-zhong, CHEN Xin（凌盛杰，邵正中，陈 新）. Process in Chemistry（化学进展）, 2014, 26(1): 178.
[49] Mourant J R, Gibson R R, Johnson T M, et al. Phys. Med. Biol., 2003, 48: 243.
[50] Miljkovic M, Romeo M, Matthus C, et al. Biopolymers, 2004, 74: 172.
[51] Marcsisin E J, Uttero C M, Miljkovic＇ M, et al. Analyst., 2010, 135(12): 3227. |
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