光谱学与光谱分析 |
|
|
|
|
|
Determination of Constituents of Polysaccharide and the Content of Saccharide in Mongolian Medicine Garidi-15 |
CUI Yi1,BAI Hai-quan2,ZHAO Yu-ying1*,Wulangerile1,SONG Juan-juan1,XU Xiu-ting1 |
1. Inner Mongolia Nationalities University, Tongliao 028043, China 2. Inner Mongolia KeYouZhongQi Teaching and Research Section, Xinganmeng 029000, China |
|
|
Abstract The present paper introuduces the method, with which the water-soluble polysaccharide was extracted with hot water from Garidi-15, the products were further purified with column chromatography on SephadexC-25, and the contents of water soluble polysaccharide were determined by phenyl hydrate-sulfuric acid method. The average recovery was 100.50%, and RSD was 0.82%. The components of water soluble polysaccharide were identified by gas chromatography as xylose, mannose, galactose and glucose in the molar ratio of 1.03∶1.26∶0.77∶2.30. The water soluble polysaccharide was identified by IR spectrum and UV(200-400 nm) scanning spectrum. The IR spectrum indicates that there are characteristic absorption peaks of polysaccharides at 3 417.46, 2 928.65, 1 742.86, 1 643.69, 1 149.78, 1 078.19, 1 022.56 and 834.57 cm-1, of which there are absorption peaks of pyranose at 1 078.19 and 1 022.56 cm-1,and contain one characteristic absorption peak of α-pyranose indican band at 834.57 cm-1. The UV scanning spectrum shows the obvious polysaccharide absorption peak at 280 nm, and indicates the existence of CO bond.
|
Received: 2006-08-06
Accepted: 2006-11-02
|
|
Corresponding Authors:
ZHAO Yu-ying
E-mail: zhaoyy1961@126.com
|
|
Cite this article: |
CUI Yi,BAI Hai-quan,ZHAO Yu-ying, et al. Determination of Constituents of Polysaccharide and the Content of Saccharide in Mongolian Medicine Garidi-15[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(11): 2246-2248.
|
|
|
|
URL: |
https://www.gpxygpfx.com/EN/Y2007/V27/I11/2246 |
[1] XIE Ming-yong, NIE Xiao-ping, FU Bo-qiang, et al(谢明勇,聂小平,傅博强,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2006,26(9):1710. [2] ZHANG Jian-hua, TIAN Geng-yuan(张健华,田庚元). Acta Chimica(化学学报),2003,61(10):1692. [3] WANG Yan, YANG Xiang-liang, DENG Cheng-hua, et al(王 雁,杨祥良,邓成华,等). Prog. Biochem. Biophgs.(生物化学与生物物理进展),2000,27(4):411. [4] Edited by Health Bureau of Inner Mongolia(内蒙古自治区卫生厅编). The Standards of Mongolian Medicine of Inner Mongolia(内蒙古蒙成药标准). Chifeng: Inner Mongolia Science and Technology Publishing House(赤峰: 内蒙科技出版社), 1984. [5] ZHAO Yu-ying, ZHAO Yu-qin, PIAO Gui-jin, et al(赵玉英, 赵玉琴, 朴贵金,等). Chinese Traditional and Herbal Drugs(中草药), 2003, 34(8): 704. [6] ZHAO Yu-ying, HAI Ping, SUN Zhan-cai, et al(赵玉英, 海 平, 孙占才, 等). Chin. J. Pharm. Anal.(药物分析杂志),2001,21(6): 440. [7] ZHAO Yu-ying(赵玉英). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2005,25(2): 314. [8] ZHAO Yu-ying, ZHAO Yu-qin, CHEN Yu-hua, et al(赵玉英, 赵玉琴, 陈玉花 等). Chinese Traditional Patent Medicine(中成药),2004,26(1): 1. [9] MENG Qing-yong, WANG Ya-fei, JIE Xin-ming, et al(孟庆勇,王亚飞,揭新明,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2006,26(10): 1903. [10] DENG Gui-chun, HOU Song-mei, TIAN Dong-mei, et al(邓桂春,侯松嵋,田东梅,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2006,26(3): 522. |
[1] |
YANG Cheng-en1, 2, LI Meng3, LU Qiu-yu2, WANG Jin-ling4, LI Yu-ting2*, SU Ling1*. Fast Prediction of Flavone and Polysaccharide Contents in
Aronia Melanocarpa by FTIR and ELM[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 62-68. |
[2] |
LI Xin-ting, ZHANG Feng, FENG Jie*. Convolutional Neural Network Combined With Improved Spectral
Processing Method for Potato Disease Detection[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 215-224. |
[3] |
ZHENG Shu-yuan1, 2, HAI Yan1, 2, HE Meng-qi1, 2, WANG Jian-xiong1, 2. Construction of Vegetation Index in Visible Light Band of GF-6 Image With Higher Discrimination[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3509-3517. |
[4] |
QIAN Rui1, XU Wei-heng2, 3 , 4*, HUANG Shao-dong2, WANG Lei-guang2, 3, 4, LU Ning2, OU Guang-long1. Tea Plantations Extraction Based on GF-5 Hyperspectral Remote Sensing
Imagery in the Mountainous Area[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3591-3598. |
[5] |
WEI Zi-kai, WANG Jie, ZHANG Ruo-yu, ZHANG Meng-yun*. Classification of Foreign Matter in Cotton Using Line Scan Hyperspectral Transmittance Imaging[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3230-3238. |
[6] |
FENG Hai-kuan1, 2, YUE Ji-bo3, FAN Yi-guang2, YANG Gui-jun2, ZHAO Chun-jiang1, 2*. Estimation of Potato Above-Ground Biomass Based on VGC-AGB Model and Hyperspectral Remote Sensing[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2876-2884. |
[7] |
WANG Jing-yong1, XIE Sa-sa2, 3, GAI Jing-yao1*, WANG Zi-ting2, 3*. Hyperspectral Prediction Model of Chlorophyll Content in Sugarcane Leaves Under Stress of Mosaic[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2885-2893. |
[8] |
DAI Shuo1, XIA Qing1*, ZHANG Han1, HE Ting-ting2, ZHENG Qiong1, XING Xue-min1, LI Chong3. Constructing of Tidal Flat Extraction Index in Coastal Zones Using Sentinel-2 Multispectral Images[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1888-1894. |
[9] |
FENG Ying-chao1, HUANG Yi-ming2*, LIU Jin-ping1, JIA Chen-peng2, CHEN Peng1, WU Shao-jie2*, REN Xu-kai3, YU Huan-wei3. On-Line Monitoring of Laser Wire Filling Welding Process Based on Emission Spectrum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1927-1935. |
[10] |
JIA Meng-meng, YIN Yong*, YU Hui-chun, YUAN Yun-xia, WANG Zhi-hao. Hyperspectral Imaging Combined With Feature Wavelength Screening for Monitoring the Quality Change of Tomato During Storage[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 969-975. |
[11] |
FENG Xin1, 2, FANG Chao1*, GONG Hai-feng2, LOU Xi-cheng1, PENG Ye1. Infrared and Visible Image Fusion Based on Two-Scale Decomposition and
Saliency Extraction[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(02): 590-596. |
[12] |
ZHANG Hai-yang, ZHANG Yao*, TIAN Ze-zhong, WU Jiang-mei, LI Min-zan, LIU Kai-di. Extraction of Planting Structure of Winter Wheat Using GBDT and Google Earth Engine[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(02): 597-607. |
[13] |
WANG Zhi-xin, WANG Hui-hui, ZHANG Wen-bo, WANG Zhong, LI Yue-e*. Classification and Recognition of Lilies Based on Raman Spectroscopy and Machine Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(01): 183-189. |
[14] |
CAI Yu1, 2, ZHAO Zhi-fang3, GUO Lian-bo4, CHEN Yun-zhong1, 2*, JIANG Qiong4, LIU Si-min1, 2, ZHANG Cong-zi4, KOU Wei-ping5, HU Xiu-juan5, DENG Fan6, HUANG Wei-hua7. Research on Origin Traceability of Rhizoma Dioscoreae Based on LIBS[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(01): 138-144. |
[15] |
YAN Wen-hao1, YANG Xiao-ying1, GENG Xin1, WANG Le-shan1, LÜ Liang1, TIAN Ye1*, LI Ying1, LIN Hong2. Rapid Identification of Fish Products Using Handheld Laser Induced Breakdown Spectroscopy Combined With Random Forest[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(12): 3714-3718. |
|
|
|
|