光谱学与光谱分析 |
|
|
|
|
|
Effect of Temperature on the Aggregation Behavior of Collagen Solution by Two-Dimensional Synchronous Fluorescence Correlation Spectroscopy |
WU Wan-ye1, WU Kun2, LI Guo-ying1,2* |
1. The Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, China 2. National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, China |
|
|
Abstract The synchronous fluorescence spectroscopy and two dimensional correlation analysis method were applied to study the aggregation behavior of acid-soluble collagen solutions (0.2, 0.4 and 1.6 mg·mL-1) during the heating process of 10~70 ℃. It was found that the fluorescence excited at 292 and 282 nm (Δλ=9 nm) belongs to the tyrosine (Tyr) residues which participate in forming hydrogen bonds or not, respectively. The two dimensional correlation analysis with the temperature varying showed that with the temperature increased (10~30 ℃) hydrogen bonds among collagen molecular with Tyr residues formed in the 0.2 mg·mL-1 collagen solution, while the higher aggregations of collagen molecular and hydrophobic micro-domains appeared in the 0.4 and 1.6 mg·mL-1 collagen solutions. With approaching the denatured temperature of collagen (36~38 ℃), the hydrophobic micro-domain and aggregates seemed to be broken in the 0.4 and 1.6 mg·mL-1 collagen solutions, however the hydrogen bonds in the 0.2 mg·mL-1 were stable. Above the denaturation temperature of collagen, the triple-helix structure of collagen molecular in solution of each concentration tended to be loose. In the heating process of 45~70 ℃, this trend was more obvious.
|
Received: 2013-11-29
Accepted: 2014-03-14
|
|
Corresponding Authors:
LI Guo-ying
E-mail: liguoyings@163.com
|
|
[1] LI Guo-ying, LIU Wen-tao(李国英,刘文涛). Collagen Chemistry(胶原化学). Beijing: China Light Industry Press(北京:中国轻工业出版社), 2013. 1. [2] Schmitt F O, Gross J, Highberger J H, Experimental Cell Research, 1955, 3: 326. [3] Wu Kun, Liu Wentao, Li Guoying. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2013, 102: 192. [4] Fan Daidi, Xing Jianyuan, Xue Wentao, et al. Chinese Journal of Chemistry, 2011, 29(9): 1811. [5] Julian M Menter. Photochem. Photobiol. Sci., 2006, 5: 403. [6] Lloyd J B F. Journal of the Forensic Science Society, 1971, 11(2): 83. [7] Digambara Patra, Mishra A K. Trends in Analytical Chemistry, 2002, 21(12): 787. [8] Liu Yongliang, Yukihiro Ozaki, Noda I. The American Journal of Physics,1996, 100(18): 7326. [9] Noda I, Dowrey A E, Marcott C, et al. Applied Spectroscopy, 2000, 54(7): 236. [10] Noda I. Applied Spectroscopy, 1993, 47(9): 1329. [11] PENG Xian-neng, CHEN Xin, WU Pei-yi, et al(彭显能,陈 新,武培怡,等). Acta Chimaca Sinaca(化学学报), 2001, 62(21): 2127. [12] Li Yang, Li Yanwen, Du Zongliang, et al. Thermochimica Acta, 2008, 469(1-2): 71. [13] Shae B Padrick, Andrew D Miranker. Journal of Molecular Biology, 2001, 308(4): 783. [14] Mu Changdao, Li Defu, Lin Wei, et al. Biopolymer, 2007, 86(4): 282. [15] Liu Wentao, Li Guoying. Polymer Degradation and Stability, 2010, 95(12): 2233. |
[1] |
ZHANG Ning-chao1, YE Xin1, LI Duo1, XIE Meng-qi1, WANG Peng1, LIU Fu-sheng2, CHAO Hong-xiao3*. Application of Combinatorial Optimization in Shock Temperature
Inversion[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3666-3673. |
[2] |
LIANG Ya-quan1, PENG Wu-di1, LIU Qi1, LIU Qiang2, CHEN Li1, CHEN Zhi-li1*. Analysis of Acetonitrile Pool Fire Combustion Field and Quantitative
Inversion Study of Its Characteristic Product Concentrations[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3690-3699. |
[3] |
YANG Ke-li1, 2, PENG Jiao-yu1, 2, DONG Ya-ping1, 2*, LIU Xin1, 2, LI Wu1, 3, LIU Hai-ning1, 3. Spectroscopic Characterization of Dissolved Organic Matter Isolated From Solar Pond[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3775-3780. |
[4] |
LI Xiao-dian1, TANG Nian1, ZHANG Man-jun1, SUN Dong-wei1, HE Shu-kai2, WANG Xian-zhong2, 3, ZENG Xiao-zhe2*, WANG Xing-hui2, LIU Xi-ya2. Infrared Spectral Characteristics and Mixing Ratio Detection Method of a New Environmentally Friendly Insulating Gas C5-PFK[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3794-3801. |
[5] |
CHEN Heng-jie, FANG Wang, ZHANG Jia-wei. Accurate Semi-Empirical Potential Energy Function, Ro-Vibrational Spectrum and the Effect of Temperature and Pressure for 12C16O[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3380-3388. |
[6] |
YU Hao-zhang, WANG Fei-fan, ZHAO Jian-xun, WANG Sui-kai, HE Shou-jie*, LI Qing. Optical Characteristics of Trichel Pulse Discharge With Needle Plate
Electrode[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3041-3046. |
[7] |
TIAN Fu-chao1, CHEN Lei2*, PEI Huan2, BAI Jie-qi1, ZENG Wen2. Diagnosis of Emission Spectroscopy of Helium, Methane and Air Plasma Jets at Atmospheric Pressure[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2694-2698. |
[8] |
ZENG Si-xian1, REN Xin1, HE Hao-xuan1, NIE Wei1, 2*. Influence Analysis of Spectral Line-Shape Models on Spectral Diagnoses Under High-Temperature Conditions[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2715-2721. |
[9] |
LI Chen-xi1, SUN Ze-yu1, 2, ZHAO Yu2*, YIN Li-hui2, CHEN Wen-liang1, 3, LIU Rong1, 3, XU Ke-xin1, 3. The Research Progress of Two-Dimensional Correlation Spectroscopy and Its Application in Protein Substances Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 1993-2001. |
[10] |
LI Chang-ming1, CHEN An-min2*, GAO Xun3*, JIN Ming-xing2. Spatially Resolved Laser-Induced Plasma Spectroscopy Under Different Sample Temperatures[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2032-2036. |
[11] |
LIANG Wen-ke, WEI Guang-fen, WANG Ming-hao. Research on Methane Detection Error Caused by Lorentzian Profile Approximation[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1683-1689. |
[12] |
WANG Pei-qi, CHENG Xiao-fang*, ZHANG De-bin. Radiation Thermometry Method Based on Intersection Capture of Spectral Distribution Curves[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1676-1682. |
[13] |
LIU Si-ran1, GONG Xin1, YAN Bi-chen2. Determining the Firing Temperature of Ancient Ceramics With FTIR Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1495-1500. |
[14] |
ZHANG Li-fang1, YANG Yan-xia1, ZHAO Guan-jia1, MA Su-xia1, GUO Xue-mao2. Comparison of Numerical Iterative Algorithms for Two-Dimensional Absorption Spectral Reconstruction[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1367-1375. |
[15] |
LIU Rong1, 2, WANG Miao-miao1, 2 , SUN Ze-yu1, 2, CHEN Wen-liang1, 2, LI Chen-xi2*, XU Ke-xin1, 2. Research on Temperature Disturbance of Glucose Solution With
Two-Trace Two-Dimensional Correlation Spectrum Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(04): 1051-1055. |
|
|
|
|