| 光谱学与光谱分析 |
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| The Analyses and Identification of Flos Rhododendri Mollis and Flos Chrysanthemi Indici Via Infrared Spectroscopy |
| JIN Zhe-xiong1, WANG Yue1,2, ZHOU Qun2, CHEN Jian-bo2, MA Fang2, SUN Su-qin2* |
1. Department of Traditional Chinese Pharmacy, Harbin University of Commerce, Harbin 150001, China
2. Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China |
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Abstract In this study, major chemical components of Flos rhododendri mollis and Flos chrysanthemi indici were characterized using Fourier transform infrared spectroscopy (FTIR). For Flos rhododendri mollis, the bands at 1 648 and 1 543 cm-1 were attributed to amide Ⅰ and amide Ⅱ, respectively, indicating that it contained proteins probably resulting in immunization. In case of Flos chrysanthemi indici, stretching vibration of CO function group was responsible for the bands at 1 734 and 1 515 cm-1, as a result of essential oils, lipids, etc. Since FTIR spectra of Flos rhododendri mollis and Flos chrysanthemi indici are almost identical and it is difficult to discriminate them, two-step identification was investigated via secondary derivative of the FTIR spectra. The bands at 1 656 and 1 515 cm-1 corresponds to flavonoides in Flos rhododendri mollis and Flos chrysanthemi indici. In the secondary derivative of the FTIR spectrum of Flos chrysanthemi indici, characteristic bands of inulin were present at 1 163, 1 077, 1 026, 986 and 869 cm-1, and therefore Flos chrysanthemi indici contained inulin as well. Tri-step identification was carried out for Flos rhododendri mollis and Flos chrysanthemi indici by means of comparing their 2D-IR correlation spectra in different wave number range. In the characteristic range of flavonoides (1 700~1 400 cm-1), Flos rhododendri mollis exhibited 3 obvious autopeaks, while 10 autopeaks were visualized in the 2D-IR correlation spectrum of Flos chrysanthemi indici Moreover, in the characteristic range of glucoside (1 250~900 cm-1), 10 and 9 autopeaks were present in the 2D-IR correlation spectra of Flos rhododendri mollis and Flos chrysanthemi indici, respectively. Therefore, the tri-step identification of FTIR is a time-saving, accurate, cost-saving and convenient method to effectively distinguish traditional Chinese medicines.
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Received: 2014-05-12
Accepted: 2014-07-15
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Corresponding Authors:
SUN Su-qin
E-mail: sunsq@tsinghua.edu.cn
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[1] WU Zheng-yi(吴征镒). Flora of Yunnan(云南植物志). Beijing: Science Press(北京：科学出版社), 1986. 4.
[2] HUANG Tai-kang(黄泰康). Modern Compendium of Materia Medica Scroller(现代本草纲目). Beijing: The Medicine Science and Technology Press of China(北京：中国医药科技出版社), 2001.
[3] CHEN Jin-ming, QIN Yan-nian, SHU Wei(陈锦名, 秦延年，舒 伟). Acta Academiae Medicinae Xuzhou(徐州医学院学报), 1981, 1: 6.
[4] XIA De-chao, YANG Tian-ming, ZHU Jing-shen, et al(夏德超，杨天明, 朱景申, 等). Journal of Chinese Medicinal Materials(中药材), 2002, 25(11): 829.
[5] Shi L P, Tian L L, Yuan J S, et al. Chin. J. Intergr. Med. Cardio./Cerebrovasc. Dis.(中西医结合心脑血管病杂志), 2005, 3(5):434.
[6] SUN Su-qin, ZHOU Qun, CHEN Jian-bo(孙素琴, 周 群, 陈建波). Analysis of Traditional Chinese Medicine by Infrared Spevtroscopy(中药红外光谱分析与鉴定). Beijing: Chemical Industry Press(北京: 化学工业出版社), 2010.
[7] LU Jin-qing, LI Jun(芦金清，李 竣). Determination of Volatile Oil and Total Flavone from Various Species of Dendranthema Morifolium in Hubei(湖北产不同品种菊花中挥发油和总黄酮测定), 2002(3).
[8] ZHANG Zhi-run(张之润). Studies on the Chemical Constituents and Quality Standard of Two Rhododendron Species(两种杜鹃花属植物化学成分及质量标准研究), Kunming Unicersity of Science and Technology(昆明科技大学),2012.
[9] XIA De-chao(夏德超). Chinese Traditional and Herbal Drugs(中药材), 2002, 25(11): 829.
[10] XU Chang-hua, ZHOU Qun, SUN Su-qin, et al(许长华, 周 群, 孙素琴, 等). Chinese Journal of Analytical Chemistry(分析化学), 2005, 33(2): 221.
[11] Noda I. Bull. Am. Phys. Soc., 1986, 31: 520.
[12] WU Ming-xia, WANG Jing-juan, ZHANG Gui-jun, et al(吴明侠, 王晶娟, 张贵君, 等). Lishizhen Medicine and Materia Medical Research(时珍国医国药),2011, 22(1)：211.
[13] CAI Ding-jian, LIU Cha-hua, ZHONG Hong-ming, et al(蔡定建, 柳茶花, 钟鸿鸣, 等). China Food Additives(中国食品添加剂), 2010, 2：143. |
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