| 光谱学与光谱分析 |
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| FTIR Microspectroscopic Investigation of the Age-Related Changes of Subchondral Bone of the Knee in Guinea Pig |
| YANG Li-ping1, LIU Jian-ling2, SONG Qing-hui1, ZHU Jia1, ZHANG Wan-qiang1, KONG Huan-yu1, ZHAO Tie-jun3* |
1. Pharmacology Laboratory of Traditional Chinese Medicine Orthopedics ＆ Traumatology，Wangjing Hosptial, China Academy of Chinese Medical Sciences, Beijing 100700, China
2. Central Laboratory of 251 Hospital of PLA，Zhangjiakou 075000，China
3. Department of the 1st Orthopaedics Bone and Joint, Wangjing Hospital, China Academy of Chinese Medicine Sciences, Beijing 100102, China |
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Abstract Fourier transform infrared (FTIR) microspectroscopy was applied to in-situ analyse the chemical change of tibial articular subchondral bone of female Hartley guinea pigs with age increase. Three infrared absorption regions (a,b,c) of trabecular bone and central marrow region of the subchondral bone were measured for guinea pigs of different ages (1 months，2 months and 3 months) using the infrared spectrum. Results show that (1) with months increasing, the total area of trabecular bone is increasing, meanwhile, the region a which is similar to normal trabecular bone spectra is decreasing, and region d waveform has the same trend as region a.(2) In the second and third month, region b & c show amide Ⅲ redshift and the red shift in region c shows a shoulder peak, showing the absorption peak intensity on behalf of nucleic acid and polysaccharide in region b & c is 7 times that in region a.(3)β glycosidic bond absorption peak appears at region c in 3 different old pigs.(4) IamideⅢ/IamideⅡ is the highest in region b in the second month but lowest in the third month; IamideⅢ/IamideⅡ reduces from a to c in the second and third month; IνsPO-2/IamideⅡ in region b & c is 7 times higher than region a in the second and third Month. These results are consistent with the regular pattern of change rule of osteoarthritis subchondral bone’s organization structure and chemical composition in different stages . Our primary result illustrated that FTIR microspectroscopy can be used for in-situ analysis of the molecular organization of subchondral trabecular bone and bone marrow. It provides reliable pathology information for osteoarthritis subchondral bone tissue at molecular level.
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Received: 2013-03-31
Accepted: 2013-06-20
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Corresponding Authors:
ZHAO Tie-jun
E-mail: wangqiangas79@sohu.com
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[1] Kwan Tat S, Lajeunesse D, Pelletier J P, et al. Best Pract Res. Clin. Rheumatol., 2010，24(1)：51.
[2] Karsdal M A, Leeming D J, Dam E B, et al. Osteoarthritis Cartilage, 2008，16(6)：638.
[3] Ding M. Acta Orthop. Suppl., 2010，81(340)：1.
[4] YANG Li-ping，ZHAO Tie-jun，LIU Jian-ling, et al(杨丽平，赵铁军，刘建玲，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2012，32(10)：2651.
[5] SHAN Peng-cheng，CAO Yong-ping(单鹏程，曹永平). Orthopedic Journal of China(中国矫形外科杂志)，2009，17(23)：1792.
[6] Hayami T, Pickarski M, Zhuo Y, et al. Bone, 2006，38(2)：234.
[7] Tomoya M, Hiroshi H, Toru O, et al. Arthritis Rheum, 2007，56(10)：3366.
[8] HUANG Ting-sheng，GUO Hua(黄廷生，郭 华). West China Medical Journal(华西医学)，2012，27(7)：1104.
[9] LU Sheng-jun，LIAO Quan-ming，LU Hou-gen(陆圣君，廖全明，鲁厚根). Int. J. Orthop(国际骨科学杂志)，2011，32(3)：167.
[10] Plepis A M G, Das-Gupta D K, Goissis G. International Symposium on Electrets, 1999, (10): 233.
[11] ZHU Shao-hua，ZHANG Hui, P P Fietzek(朱少华，张 慧, P P Fietzek). Chinese Journal of Control of Endemic Diseases(中国地方病防治杂志)，2004，19(1)：11.
[12] WANG Jian-sheng，ZHANG Jia，WU Wen-an, et al(王健生，张 佳，吴文安，等). Chemical Journal of Chinese Universities(高等学校化学学报)，2010，31(3)：484.
[13] Benedetti E，Teodori L, Trinca M L, et al. Applied Spectroscopy，1990，44(1)：276.
[14] Mallen C D，Peat G, Porcheret M. Br. J. Gen. Pract., 2008, 58(554): 649. |
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