大鼠死后脑组织FTIR光谱变化与死亡时间的关系

doi:10.3964/j.issn.1000-0593(2008)11-2545-05

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Abstract In the present study, FTIR spectroscopy was applied to observe the process of postmortem degradation in rat’s brain and a lot of useful data for the estimation of postmortem interval (PMI) were provided. Sprague-Dawley male rats (weight 240±10 g) were chosen and sacrificed by cervical dislocation. The bodies were kept in a controlled environmental chamber set at (20±2) ℃, and their brain cortex as the measuring sample which was extracted at 0, 12, 24, 48, 72, 96, 120, 144 and 168 h from the 8 rats. The tissues were freeze dried in a vacuum at -50 ℃ overnight in order to dehydrate them. About 2 mg freeze dried tissues were mixed with 200 mg KBr and then ground in an agate mortar for 5 min. The mixture then was pressed into a pellet with a thickness of 0.4 mm and a diameter of 12 mm. The FTIR spectra were quantitatively recorded at room temperature in the range 4 000-400 cm^-1 on a Shimadzu 8400S spectrometer (Shimadzu, Japan). IR solution 1.10 software (Shimadzu, Japan) was used for the analysis of the FTIR spectra and for recording the data from the spectra. With the PMI increasing, the peak position of main absorbance bands in the FTIR spectra showed no significant difference, but there was a dramatic variance in the intensity: (1) The relative intensity at 1 080 and 1 238 cm^-1(I_{1 080}/I_{1 398} and I_{1 238}/I_{1 398}) related with nucleic acid tended to decrease obviously. (2) The intensity ratio at amide Ⅱ and Ⅰ(I_{1 541}/I_{1 647}) increased with the PMI increasing. The intensity at 1 338 and 1 313 cm^-1 varied slightly, but their intensity ratio to 1 398 cm^-1 decreased. (3) The intensity at 1 456 and 1 398 cm^-1 showed a trend of decreasing and increasing respectively. (4) Compared with the intensity at 2 871 cm^-1,the intensity at 2 852, 2 871, 2 923 and 2 958 cm^-1 tended to increase, but the increasing tendency at 2 871 cm^-1 was slight. It is concluded that FTIR spectroscopy is going to be an effective method for estimating the PMI in medicolegal practice and the brain tissue may be a suitable marker as a potential sample.

Key words： FTIR;PMI;Brain tissue;Chemical group

Received: 2007-07-16 Accepted: 2007-10-18

ZTFLH:

O657.3

Corresponding Authors: WANG Zhen-yuan E-mail: wzy218@mail.xjtu.edu.cn

Cite this article:

KE Yong,WANG Shi-wei,LU Qing-yang, et al. The Correlation between Postmortem Interval and Fourier Transform Infrared Spectra in Rat’s Brain[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(11): 2545-2549.

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https://www.gpxygpfx.com/EN/10.3964/j.issn.1000-0593(2008)11-2545-05 OR https://www.gpxygpfx.com/EN/Y2008/V28/I11/2545

[1] Knight B. Simpson’s Forensic Medicine. London: Oxford University Press, 1996.
[2] ZHAO Zi-qin(赵子琴). Forensic Pathology, the Third Version(法医病理学, 第3版). Beijing: People’s Medical Publishing House(北京: 人民卫生出版社), 2004.
[3] Bocaz-Beneventi G, Tagliaro F, Bortolotti F, et al. Legal Med., 2002，16: 5.
[4] YANG Jiao-lan, LUO Tian(杨娇兰，罗添). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2002，22(4): 610.
[5] LING Xiao-feng, XU Zhi, XU Yi-zhuang, et al(凌晓锋，徐智，徐怡庄，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005, 25(2): 198.
[6] WU Jin-guang(吴瑾光). The Techniques and Application of Modern Fourier Transform Infrared Spectroscopy(近代傅里叶变换红外光谱技术及应用). Beijing: Literature Press of Science and Technology(北京: 科学技术文献出版社)，1994.
[7] Mantsch H H, Chapman D. Infrared Spectroscopy of Biomolecules. New York: John Wiley, 1996.
[8] FAN Xiao-yan(范晓燕). Life Science Research(生命科学研究)，2003, 7(2): 84.
[9] Stuart B. Infrared Spectroscopy: Fundamentals and Applications. New York: John Wiley & Sons, 2004.
[10] SHEN Shi-jie, LIU Bing-yu, LI Qing, et al(沈世杰, 刘炳玉, 李清, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2000, 20(1): 28.
[11] GU Zhi-yuan(谷志远). The Modern Medical Molecular Biology(现代医学分子生物学). Beijing: People’s Military Medical Press(北京: 人民军医出版社), 1998.
[12] ZHOU Su, XU Zhi, LING Xiao-feng, et al(周苏，徐智，凌晓峰, 等). Chinese Journal of Oncology(中华肿瘤杂志)，2006，28(7): 512.
[13] TANG Wei-yue, LIU Ren-ming, LI Yun-tao, et al(唐伟跃, 刘仁明, 李云涛,等). Laser & Infrared(激光与红外)，2006，36(7): 577.
[14] YAN Xin-hui, TANG Wei-yue, HOU Xiao-qiang, et al(闫新惠, 唐伟跃, 侯晓强, 等). Laser & Infrared(激光与红外)，2007，37(3): 240.
[15] Taillandier E, Liquier F. Methods Enzymol., 1992, 211: 307.
[16] Laura A Johnson，James A J Ferris. Forensic Science International，2002，126(1): 43.
[17] Surewicz W K, Mantsch H H. Biochim. Biophys. Acta, 1988, 952(2): 115.
[18] Enzo Benedetti, Federico Papineschi, Piergiorgio Vergamini, et al. Leukemia Research, 1984, 8(3): 483.
[19] Mantsch H H, Choo-Smith L, Shaw R A. Vibrational Spectroscopy, 2002, 30: 31.
[20] Zieba-Palus J, Kunicki M. Forensic Sci. Int., 2006, 158: 164.
[21] HUANG Ping, YANG Guang-de, TUO Ya, et al(黄平, 杨广德, 托娅, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(6): 1074.
[22] HUANG Ping, TIAN Wei-ping, YANG Guang-de, et al(黄平, 田卫平, 杨广德, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(10): 1962.