| 光谱学与光谱分析 |
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| Ultraviolet Absorption Spectrum Analysis and Identification of Medicinal Plants of Paris |
| ZHANG Jin-yu1, WANG Yuan-zhong1, ZHAO Yan-li1, YANG Shao-bing1, ZHANG Ji1, YUAN Tian-jun2, WANG Jia-jun3, JIN Hang1* |
1. Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650223, China
2. Research and Development Department Yunnan Reascend Tobacco Technology (Group) Co. Ltd., Kunming 650106, China
3. Research and Development Department Hong Yun Hong He Tobacco (Group) Limited Liability Company, Kunming 650000, China |
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Abstract Species of Paris are important medicinal plants of China. They possess anticancer, hot alexipharmic, detumescence, acesodyne, and arrest blood and remove blood stasis effects. They are the main raw material for several Chinese patent drugs such as “Yunnan Baiyao”, “Gong Xue Ning”, “Re Du Qing” and “Ji De Sheng Sheyaopian”. The present paper, through optimizing the chloroform, absolute ethyl alcohol and water extraction condition of Paris by orthogonal test L43 (16), using mean value, smoothness and second differential methods on the observed UV spectrum, to inspects the RSD of stability and repeatability of different waveband. By SIMCA and the common and variant peak ratio dual index sequence analysis method, it evaluated the quality and quantity of Paris. The results showed that at the time of 50, 40 and 50 min, chloroform, absolute ethyl alcohol and water had the highest extraction ratios. Within 20 h, the RSDs of stability were 0.06～1.88, 0.05～2.42 and 0.03 ～0.35; the RSDs of accuracy were 0～1.48, 0.05～0.37 and 0.09～0.44; and the RSDs of repeatability were 0～1.23, 0.04～0.30 and 0.12～0.25 respectively. The qualitative analysis revealed large differences between different Paris species and different areas. The quantitative analysis indicated that the highest common peak ratio among the Paris samples was 80.00% and the lowest variant peak ratio was 6.25%. The method evaluated Paris of different species and from different producing areas, and also quantitatively assessed the arbitrary two samples, clarified the similarity between the species and areas of Paris, which provided basis of distinguishing the real and false, identification of variety and quality evaluation for Chinese herbal medicine.
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Received: 2012-02-01
Accepted: 2012-04-17
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Corresponding Authors:
JIN Hang
E-mail: jinhang2009@126.com
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