| 光谱学与光谱分析 |
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| FTIR Analysis of the Impact of Covalent Coupling on the Secondary Structure of Antibody Protein |
| YU Si-ming1, PENG Yun-ping1, 2, YU Shu-juan1*, Lü Huan1 |
1. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China
2. Wondofo Biotech Co., Ltd., Guangzhou 510640, China |
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Abstract The immunolatex was prepared by covalent coupling. FTIR technology combined with substractive spectroscopy, deconvolution, derivation and curve-fitting methods were used to study the structure of the antibody protein on the immunolatex. The result demonstrates that the α-helix strcture of antibody increases with the increase in the pH value and the concentration of latex. So it is concluded that covalent coupling has a great impact on the secondary structure of antibody protein.
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Received: 2011-07-06
Accepted: 2011-10-29
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Corresponding Authors:
YU Shu-juan
E-mail: lfshjyu@scut.edu.cn
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