| 光谱学与光谱分析 |
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| Resonance Light Scattering Study on Interaction of Solochrome Cyanine R with Protein and Light Scattering Determination of Trace Protein |
| FENG Ning-chuan，HE Su-ping，ZHANG Juan，LIU Jian-ping |
| Department of Chemistry, Ningxia Medical College, Yinchuan 750004，China |
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Abstract The resonance light scattering (RLS) of Solochrome Cyanine R(SCR) is greatly enhanced by proteins. Based on this phenomenon, a novel method for the determination of protein by using SCR as a labeling agent was developed. In the pH 4.0 solution the enhanced intensity of RLS at 400 nm is proportional to the concentration of protein. The linear range for bovine serum albumin (BSA) is 0-5.0 mg·L-1 and the limit of detection is 44.4 μg·L-1. The method is simple, rapid, sensitive, stable，and tolerant of many foreign substances. It has been used to determine proteins in human urine samples with satisfactory results.
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Received: 2003-01-18
Accepted: 2003-06-06
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Corresponding Authors:
FENG Ning-chuan
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| Cite this article: |
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FENG Ning-chuan,HE Su-ping,ZHANG Juan, et al. Resonance Light Scattering Study on Interaction of Solochrome Cyanine R with Protein and Light Scattering Determination of Trace Protein [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(02): 194-196.
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| URL: |
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http://www.gpxygpfx.com/EN/Y2004/V24/I02/194 |
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