| 光谱学与光谱分析 |
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| Study on the Interaction of Cystine and Polyphenol Oxidase from Nicotian Tobaccum |
| XIAO Hou-rong，XU Xiao-long，XIE Yong-shu，ZHANG Yan-ge，PENG Dun-geng，LIU Qing-liang* |
| Department of Chemistry, University of Science and Technology of China, Hefei 230026, China |
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Abstract The interaction of cystine and polyphenol oxidase(PPO) from nicotian tobaccum has been studied. The results show that cystine has an inhibitory effect on the enzymatic activity, the mechanism of which might be that the thiolether in cystine coordinates with Cu2+ in the active site of PPO, and the inactivation constant value of PPO by cystine is 0.633 min-1, while the substrates or the products inhibit their combination. Cystine can also be combined with the products of enzymatic reaction to form a colourless compound. Cystine can inhibit the enzymatic activity completely when the molar ratio of cystine to PPO approaches 16 000∶1. The effect of cystine on the fluorescence changes with the molar ratio of cystine to the PPO. However, when the molar ratio gets to 75∶1, cystine has no longer the effect on either the fluorescence spectra or the synchronous spectra. Microenvironment of trp residue in PPO is more hydrophobic than that of free trp in water.
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Received: 2003-09-20
Accepted: 2004-02-08
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Corresponding Authors:
LIU Qing-liang
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| Cite this article: |
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XIAO Hou-rong,XU Xiao-long,XIE Yong-shu, et al. Study on the Interaction of Cystine and Polyphenol Oxidase from Nicotian Tobaccum [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(12): 1618-1622.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2004/V24/I12/1618 |
[1] HUANG Ming, PENG Shi-qing(黄 明, 彭世清). Journal of Guangxi Normal University(广西师范大学学报), 1998, 16(2): 65.
[2] Victoria Martinez M，John R Whitaker. Trends in Food Science and Technology, 1995, 6: 195.
[3] Osamu Nwgishi, Tesuo Ozawa. Phytochemistry, 2000, 54: 481.
[4] LIN Jian-qiao, WANG Wei-jun, MU Hong, XU Feng-cai(林健巧, 王炜军, 穆 虹, 徐风彩). Chin. J. of Biochem. Mol. Biol.(中国生物化学与分子生物学报)，1999, 15(4): 663.
[5] CHOU Ju, QU Xiao-gang, LU Tian-hong, WU Yue(侴 菊, 曲晓刚, 陆天虹, 吴 越). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 1997, 17(2): 108.
[6] CHOU Ju, DU Jiang-yan, FENG Yu-ying, YANG Xiu-juan, LU Tian-hong(侴 菊, 杜江燕, 冯玉英, 杨秀娟, 陆天虹). Chinese Journal of Analytical Chemistry(分析化学), 2001, 29(2): 219.
[7] SHI Chun-hua, DAI Ya, XU Xiao-long, XIE Yong-shu，LIU Qing-liang. Protein Expression and Purification, 2002, 24: 51.
[8] Sanda H, Suzue R, Nakashima Y, Kawad S. Biochim. Biophys. Acta, 1972, 1261: 258.
[9] Richard-Forget F C, Goupy P M, Nicolas J J, Lacombe M J，Pavia A A. J. Agric. Food Chem., 1991, 39: 481.
[10] Christine Robert, Claude Rouch, Frederic Cadet. Food Chemistry, 1997, 59: 355.
[11] Prota G. In Natural Sulfur Compounds(Edited by Cavanilli D, Gauli E， Zappia V.). New York: Plenum Press，1980: 391.
[12] Dawson C R，Magee R J. Meth. Enzymol.，1965, 5: 817.
[13] Christine Robert, Richard-Forget F C, Claude Rouch, Michel Pabion, Frederic Cadet. J. Biochem. Cell Biol., 1996, 28(4): 457.
[14] GUO Yao-jun(郭尧君). Spectrophotometric Technology and Its Application in Biochemistry(分光光度技术及其在生物化学中的应用). Beijing：Science Press(北京: 科学出版社), 1987. 230.
[15] TAO Wei-sun, LI Wei, JING Yong-ming(陶慰孙, 李 惟, 姜涌明). The Molecular Basis of Protein, 2nd ed(蛋白质分子基础·第2版). Beijing: Higher Education Press(北京：高等教育出版社), 1995. 122.
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