| 光谱学与光谱分析 |
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| Study of Solid-Phase Time-Resolved Fluorescence Label Immunoassay |
| PAN Li-hua1, ZHOU Shi-hong1，SUN Wen-wei2，XIE Wen-bing1，ZHAO Chao1 |
1. National Analytical Research Center of Electrochemistry and Spectroscopy, Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
2. China-japan Union Hospital of Jilin University, Changchun 130031, China |
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Abstract This paper describes optimal conditions for HBsAbIgG labeling with a new fluorescence probe, 4,7-bis-chorosulfophenyl-1,10-phenanthroline-2,9-dicarboxylic acid(BCPDA)for the solid phase time-resolved fluorimmunoassay (TRFIA). The result of experiment under states clearly that BCPDA may react with protein under relative mild condition. The relative bioactivity of reacted protein was more than 80%. The labeling molar ratio of BCPDA for HBｓAbIgG was 45-70. The recovery was higher than 80%. Protein-BCPDA-Eu3+ complex is stable. It can emit very high fluorescence intensity with very long fluorescence life times. The fluorescence of Protein-BCPDA-Eu3+ complex has a very large stokes shift (270 nm). The emission band at 611.2 nm is very narrow. The research provides the base for developing non-isotopic immunoassay technique and clinical medical diagnosis.
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Received: 2003-02-08
Accepted: 2003-06-26
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Corresponding Authors:
PAN Li-hua
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| Cite this article: |
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PAN Li-hua,ZHOU Shi-hong,SUN Wen-wei, et al. Study of Solid-Phase Time-Resolved Fluorescence Label Immunoassay [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(12): 1601-1604.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2004/V24/I12/1601 |
[1] Soini E, Hemmila I. Clin. Chem.，1979, 25: 353.
[2] Hemmila I. Clin. Chem.，1985, 31：359.
[3] Evangelista R A, Pollak A, Allore B et al. Clin. Biochem., 1988，21：173.
[4] Mathis G. J. Clin. Ligand Assay．，1997, 20：141.
[5] Yuan Jingli，Matsumoto Kazuko, Kimura Hiroko. Anal. Chem．，1998, 70：596.
[6] PAN Li-hua，GAO Min, LIN Min, DING Chun-li, SUN Wen-wei, ZHAO Jie(潘利华，高 敏，林 敏，丁春黎，孙文伟，赵 劼). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2002，22(4)：573.
[7] PAN Li-hua, ZENG Chun-hui, GUAN Ming, ZHAO Chao, LIN Min, XIE Wen-bing(潘利华，曾春慧，关 铭，赵 超，林 敏，谢文兵). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003，23(5)：903.
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